separation science and proteomics

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Separation Science and Proteomics
Author : Alexandre Stoyanov
Publisher : Academic Press
Release Date : 2017-06-15
ISBN 10 : 9780128053966
Pages : 250 pages
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Separation Methods In Proteomics
Author : Gary B. Smejkal,Alexander Lazarev
Publisher : CRC Press
Release Date : 2005-12-12
ISBN 10 : 1420028057
Pages : 536 pages
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Driven by the widespread growth of proteomic practices, protein separation techniques have been refined to minimize variability, optimize particular applications, and adapt to user preferences in the analysis of proteins. Separation Methods in Proteomics provides a comprehensive examination of all major separation techniques for proteomics research. Written as a compilation of hands-on methods exemplified by the work of several recognized leaders in the field, this book may serve as a guide for selection of the optimal separation strategies to solve particular biological problems. Recent progress in the development of robust analytical techniques and instrumentation has created the need for good quality biological samples that are subject to analysis. Emphasizing the importance of sample preparation, the book explains how proteomes can be divided into smaller, less complicated “subproteomes" for individual analysis. It also highlights several hybrid approaches that take into account protein interactions. Including applications of the separation methods currently employed in proteomic analyses for both clinical and basic research, Separation Methods in Proteomics contains practical information that can enhance the current and future endeavors of scientists in proteomics, genomics, transcriptomics, biomarker discovery, and drug discovery.

Polymer Monoliths Photopatterned Within Microfluidic Devices for Proteomics and Separation Science
Author : Mei He
Publisher : N.A
Release Date : 2008
ISBN 10 :
Pages : 322 pages
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Handbook of Isoelectric Focusing and Proteomics
Author : David Garfin,Satinder Ahuja
Publisher : Academic Press
Release Date : 2005-05-23
ISBN 10 : 9780120887521
Pages : 334 pages
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Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool for protein purification. The only current book on the market, the Handbook of Isoelectric Focusing and Proteomics is the ideal 'one-stop' source for germane information in this discipline. This highly practical book also contains chapters on alternative methods that may pave the way in the search for efficient techniques for fractionating and purifying proteins. Complete with the history of IEF focusing to authors' insights and practical tips, this book is a must for anyone working in proteomics. * Is the only current book available on the subject * Includes author insights and practical tips * Is an ideal single source for students and researchers working in proteomics

Introducing Proteomics
Author : Josip Lovric
Publisher : John Wiley & Sons
Release Date : 2011-06-17
ISBN 10 : 1119957192
Pages : 296 pages
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Introducing Proteomics gives a concise and coherent overview of every aspect of current proteomics technology, which is a rapidly developing field that is having a major impact within the life and medical sciences. This student-friendly book, based on a successful course developed by the author, provides its readers with sufficient theoretical background to be able to plan, prepare, and analyze a proteomics study. The text covers the following: Separation Technologies Analysis of Peptides/Proteins by Mass Spectrometry Strategies in Proteomics This contemporary text also includes numerous examples and explanations for why particular strategies are better than others for certain applications. In addition, Introducing Proteomics includes extensive references and a list of relevant proteomics information sources; essential for any student. This no-nonsense approach to the subject tells students exactly what they need to know, leaving out unnecessary information. The student companion site enhances learning and provides answers to the end of chapter problems. "I think this book will be a popular and valuable resource for students and newcomers to the field who would like to have an overview and initial understanding of what proteomics is about. The contents are well organized and address the major issues." —Professor Walter Kolch, Director, Systems Biology Ireland & Conway Institute, University College Dublin Companion Website www.wiley.com/go/lovric

Issues in Chemical, Biological, and Medical Engineering: 2013 Edition
Author : N.A
Publisher : ScholarlyEditions
Release Date : 2013-05-01
ISBN 10 : 1490108920
Pages : 660 pages
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Issues in Chemical, Biological, and Medical Engineering: 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Biosystems Engineering. The editors have built Issues in Chemical, Biological, and Medical Engineering: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Biosystems Engineering in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in Chemical, Biological, and Medical Engineering: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Proteomics Today
Author : Mahmoud H. Hamdan,Pier G. Righetti
Publisher : John Wiley & Sons
Release Date : 2005-05-13
ISBN 10 : 0471709107
Pages : 448 pages
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The last few years have seen an unprecedented drive toward theapplication of proteomics to resolving challenging biomedical andbiochemical tasks. Separation techniques combined with modern massspectrometry are playing a central role in this drive. This bookdiscusses the increasingly important role of mass spectrometry inproteomic research, and emphasizes recent advances in the existingtechnology and describes the advantages and pitfalls as well. * Provides a scientifically valid method for analyzing theapproximatey 500,000 proteins that are encoded in the humangenome * Explains the hows and whys of using mass spectrometry inproteomic analysis * Brings together the latest approaches combining separationtechniques and mass spectrometry and their application in proteomeanalysis * Comments on future challenges and how they may be addressed * Includes sections on troubleshooting

Gel-free Proteomics
Author : N.A
Publisher : N.A
Release Date : 2009
ISBN 10 :
Pages : 137 pages
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Proteome Characterization and Proteomics
Author : Timothy D. Veenstra,Richard D. Smith
Publisher : Academic Press
Release Date : 2003-10-01
ISBN 10 : 9780080569154
Pages : 413 pages
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The content of this volume is designed to reach a wide audience, including those involved with relevant technologies such as electrophoresis and mass spectrometry, to those interested in how proteomics can benefit research. A wide range of techniques are discussed, each specifically designed to address different needs in proteomic analysis. The concluding chapter discusses the important issue related to handling large amounts of data accumulated in proteomic studies. Discusses proteomics in the postgenomic age Includes various strategies for quantitative proteomics Covers the role of MS in structural functional proteomics and proteomics in drug discovery and bioinformatics

Monolithic Silicas in Separation Science
Author : Klaus K. Unger,Nobuo Tanaka,Egidijus Machtejevas
Publisher : John Wiley & Sons
Release Date : 2010-11-29
ISBN 10 : 352763326X
Pages : 362 pages
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Edited by the experts and pioneers in the field, this is the first monograph to cover the topic, containing the must-have information hitherto only scattered among journals. Clearly divided into sections on preparation, characterization and modeling, and applications, this is essential reading for chemists, chromatographers, analytical chemists, biochemists and biologists.

Proteomics of Human Body Fluids
Author : Visith Thongboonkerd
Publisher : Springer Science & Business Media
Release Date : 2008-01-23
ISBN 10 : 9781597454322
Pages : 533 pages
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This volume is the first collection of applications of proteomics to analyze various human body fluids. Proteomics of Human Bodyfluids consists of two parts. The first provides basic principles and strategies for proteomic analysis of human body fluids. The second offers more details regarding methodologies and recent findings and clinical applications of each specific type of human body fluids.

A Century of Separation Science
Author : Haleem J. Issaq
Publisher : CRC Press
Release Date : 2001-12-19
ISBN 10 : 1482270927
Pages : 776 pages
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A Century of Separation Science presents an extensive overview of the critical developments in separation science since 1900, covering recent advances in chromatography, electrophoresis, field-flow fractionation, countercurrent chromatography, and supercritical fluid chromatography for high-speed and high-throughput analysis.

Proteomics: Biomedical and Pharmaceutical Applications
Author : Hubert Hondermarck
Publisher : Springer Science & Business Media
Release Date : 2004-08-10
ISBN 10 : 1402023227
Pages : 396 pages
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Human biology has now entered into a phase of post-genomics and it might not be an exaggeration to say that the major outcome of the human genome sequencing has finally been to open the way to the exploration of the proteome-proteomics. Proteins are the functional output of genes and there are two main expected outcomes from human proteomics. The first is to discover new molecular markers for early diagnosis and profiling of pathologies. The second is to decipher the intracellular signaling pathways leading to the initiation and progression of pathologies, for the identification of new targets and the development of innovative therapeutic strategies. This is clearly a promising challenge that this book explores through a series of ongoing experiences and projects representative of the new era in which biology and medicine have now entered. This volume will be of particular interest to students and professionals engaged in the fields of medicine, biomedical and biopharmaceutical sciences.

Separation Methods In Proteomics
Author : Gary B. Smejkal,Alexander Lazarev
Publisher : CRC Press
Release Date : 2005-12-12
ISBN 10 : 9780824726997
Pages : 536 pages
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Driven by the widespread growth of proteomic practices, protein separation techniques have been refined to minimize variability, optimize particular applications, and adapt to user preferences in the analysis of proteins. Separation Methods in Proteomics provides a comprehensive examination of all major separation techniques for proteomics research. Written as a compilation of hands-on methods exemplified by the work of several recognized leaders in the field, this book may serve as a guide for selection of the optimal separation strategies to solve particular biological problems. Recent progress in the development of robust analytical techniques and instrumentation has created the need for good quality biological samples that are subject to analysis. Emphasizing the importance of sample preparation, the book explains how proteomes can be divided into smaller, less complicated “subproteomes" for individual analysis. It also highlights several hybrid approaches that take into account protein interactions. Including applications of the separation methods currently employed in proteomic analyses for both clinical and basic research, Separation Methods in Proteomics contains practical information that can enhance the current and future endeavors of scientists in proteomics, genomics, transcriptomics, biomarker discovery, and drug discovery.

Low-Abundance Proteome Discovery
Author : Egisto Boschetti,Pier Giorgio Righetti
Publisher : Newnes
Release Date : 2013-03-26
ISBN 10 : 0124045960
Pages : 364 pages
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Low-Abundance Proteome Discovery addresses the most critical challenge in biomarker discovery and progress: the identification of low-abundance proteins. The book describes an original strategy developed by the authors that permits the detection of protein species typically found in very low abundance and that may yield valuable clues to future discoveries. Known as combinatorial peptide ligand libraries, these new methodologies are one of the hottest topics related to the study of proteomics and have applications in medical diagnostics, food quality, and plant analysis. The book is written for university and industry scientists starting proteomic studies of complex matrices (e.g., biological fluids, biopsies, recalcitrant plant tissues, foodstuff, and beverage analysis), researchers doing wet chemistry, and graduate-level students in the areas of analytical and biochemistry, biology, and genetics. Covers methodologies for enhancing the visibility of low-abundance proteins which, until now, has been the biggest challenge in biomarker progress Includes detailed protocols that address real-life needs in laboratory practice Addresses all applications, including human disease, food and beverage safety, and the discovery of new proteins/peptides of importance in nutraceutics Compiles the research and analytic protocols of the two scientists who are credited with the discovery of these landmark methodologies, also known as combinatorial peptide ligand libraries, for the identification of low-abundance proteins

Design and Synthesis of Advanced Materials for Separation Science
Author : Michael Douglas Slater
Publisher : N.A
Release Date : 2009
ISBN 10 :
Pages : 380 pages
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Proteomic and Metabolomic Approaches to Biomarker Discovery
Author : Haleem J. Issaq,Timothy D. Veenstra
Publisher : Academic Press
Release Date : 2019-10-24
ISBN 10 : 0128197889
Pages : 504 pages
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Proteomic and Metabolomic Approaches to Biomarker Discovery, Second Edition covers techniques from both proteomics and metabolomics and includes all steps involved in biomarker discovery, from study design to study execution. The book describes methods and presents a standard operating procedure for sample selection, preparation and storage, as well as data analysis and modeling. This new standard effectively eliminates the differing methodologies used in studies and creates a unified approach. Readers will learn the advantages and disadvantages of the various techniques discussed, as well as potential difficulties inherent to all steps in the biomarker discovery process. This second edition has been fully updated and revised to address recent advances in MS and NMR instrumentation, high-field NMR, proteomics and metabolomics for biomarker validation, clinical assays of biomarkers and clinical MS and NMR, identifying microRNAs and autoantibodies as biomarkers, MRM-MS assay development, top-down MS, glycosylation-based serum biomarkers, cell surface proteins in biomarker discovery, lipodomics for cancer biomarker discovery, and strategies to design studies to identify predictive biomarkers in cancer research. Addresses the full range of proteomic and metabolomic methods and technologies used for biomarker discovery and validation Covers all steps involved in biomarker discovery, from study design to study execution Serves as a vital resource for biochemists, biologists, analytical chemists, bioanalytical chemists, clinical and medical technicians, researchers in pharmaceuticals and graduate students

Proteomics and Peptidomics
Author : Gyorgy Marko-Varga
Publisher : Elsevier
Release Date : 2005
ISBN 10 : 044451810X
Pages : 632 pages
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Proteomics and peptidomics is the detailed understanding of the role that proteins and peptides play in health and disease and is a necessary compliment to genetic analysis. The functional expression analysis of both proteins and peptides plays a central role in modern drug discovery as well as drug development, and is also a key research area in systems biology.Proteomics and Peptidomics captures the width as well as the depth within the area and exemplifies the variety as well as the traditional basis of analytical chemistry that is needed in order to move forward in expression analysis studies. As a fast emerging field, it gives and overview of parts within the field combined with highly specialized and dedicated topics that are intended to compliment each other.

Handbook of Isoelectric Focusing and Proteomics
Author : David Garfin,Satinder Ahuja
Publisher : Elsevier
Release Date : 2005-09-29
ISBN 10 : 0080533620
Pages : 380 pages
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Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool for protein purification. The only current book on the market, the Handbook of Isoelectric Focusing and Proteomics is the ideal 'one-stop' source for germane information in this discipline. This highly practical book also contains chapters on alternative methods that may pave the way in the search for efficient techniques for fractionating and purifying proteins. Complete with the history of IEF focusing to authors' insights and practical tips, this book is a must for anyone working in proteomics. * Is the only current book available on the subject * Includes author insights and practical tips * Is an ideal single source for students and researchers working in proteomics

Proteomics in Practice
Author : Reiner Westermeier,Tom Naven,Hans-Rudolf Höpker
Publisher : John Wiley & Sons
Release Date : 2008-09-08
ISBN 10 : 3527622306
Pages : 502 pages
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Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR. Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.