Investigation of muscle diseases has changed dramatically with the understanding of genetic basis of a wide range of muscle diseases. Muscle biopsy has become a powerful tool not only to provide diagnosis but to make tissue available for genetic studies and to basic scientists for biomedical research. Accurate interpretation of muscle biopsy to detect cell dysfunction/ damage/death or absence / abnormality of a protein or genetic defect by the sophisticated technologies is important to guide treatment of various muscle diseases. In this book on muscle biopsy various chapters deal with the procedure and interpretation of muscle biopsy, its use in the culture of myotubes and membrane transport studies.Muscle biopsy is an important technique to investigate mitochondrial dysfunction and the mitochondrial DNA integrity in oxidation. Phosphorylation in various metabolic diseases like obesity, type 2 diabetes mellitus and peripheral vascular disease is explored in the other chapters with detailed descriptions on methodology. This book provides the advances in the basic techniques of muscle biopsy for a neuroscientist.
Muscle Biopsy: A Practical Approach gives you all of the unparalleled guidance necessary to effectively interpret and diagnose muscle biopsy specimens for the full range of diseases in both adults and children. Authored by Dr. Victor Dubowitz, an internationally renowned figure in the field of muscle disease, this medical reference book takes an integrated approach to diagnosis and assessment of muscle biopsies that includes clinical, genetic, biochemical, and pathological features. It's the comprehensive, up-to-date coverage you need to evaluate muscle disorders with confidence. Consult this title on your favorite e-reader, conduct rapid searches, and adjust font sizes for optimal readability. Bridge the gap between clinical syndromes/disorders and their underlying pathologies with the guidance of muscle disease expert, Dr. Victor Dubowitz, who skillfully guides you through the complexities of pathologic diagnoses and their implications for clinical treatment. Understand and apply expert techniques for obtaining a muscle biopsy, and familiarize yourself with the histochemical, histological, electron microscopical, and molecular appearance of normal muscle and the pathology of individual muscle disease. Apply all of the latest diagnostic techniques for neurodegenerative and genetic diseases with a brand-new chapter on myopathies associated with systemic disorders and aging, and use advanced techniques such as immunohistochemistry and immunoblotting to produce the most accurate diagnoses possible for a full range of muscle disorders. Stay current in practice with state-of-the-art coverage of genetic markers for individual conditions and antibodies used in immunocytochemical diagnosis. Understand the genetics of muscular dystrophies with absolute clarity through the use of brilliantly simple diagrams and tables, and compare your specimens to a wealth of superb color images capturing the full spectrum of muscle biopsy findings. Take advantage of international insights and fresh perspectives in muscle diseases and disorders from new author Dr. Anders Oldfors, from the Department of Pathology, University of Goteborg, Sweden.
|Author||: Victor Dubowitz,Caroline A. Sewry,Anders Oldfors,Russell J. M. Lane|
|Publisher||: Elsevier Health Sciences|
|Release Date||: 2013|
|ISBN 10||: 0702043400|
|Pages||: 572 pages|
Muscle Biopsy: A Practical Approach serves as a succinct but comprehensive reference for pathologists and neurologists in practice and in training that skilfully guides them through the complexities of diagnosing muscle biopsy specimens and highlights their implications for clinical treatment. the authors are all internationally known for their work on muscle disease and this practical reference work successfully bridges the gap between clinical syndromes and disorders and their underlying pathologies. This practical text distils a wealth of experience by focusing on the most important element
Museie biopsy is a long-established technique in clinical practice having been introduced by Duchenne in 1868 (Arch. Gen. Med. , 11, 5-179). However, the needle method used by Duchenne was not generally adopted, although Shank and Hoagland described a similar technique in 1943 (Science, 98, 592), and open muscle biopsy has for long been preferred in clinical practice, even with the advent of newer needle biopsy methods (Bergstrom, 1962, Scand. J. Clin. Lab. Invest. , 14, Suppl. 68, 1-110). The development of enzyme histochemical techniques has contributed greatly to knowledge of muscle pathology. More recently electron microscopy and immunocytochemistry have also been applied to clinical diagnosis of neuromuscular disease. This book is intended to serve as a practical guide in muscle pathology, particularly for histopathologists, and for those in training. As enzyme histochemistry has become more widely available, formalin-fixed methods have become less frequently used in muscle biopsy work. In this new edition of Muscle Biopsy Pathology we have taken account of the advances in classification and histological technique, and in knowledge of neuromuscular diseases, that have emerged since the first editionwas published in 1984. We hope that this book will continue to be used as a practical guide in the diagnosis and understanding of these disorders. 1. Introduction 1. 1 Generalfeatures of muscle The differentiation of musde into red and white types is a feature of all vertebrates and, indeed, of chordates.
A Certain Kind of Wisdom In Plato’s Apology, the Greek philosopher Socrates is on trial to defend himself against the allegation of corrupting the youth of Athens. Socrates denies this charge and offers an alternate reason for why he is on trial. He explains, “[w]hat has caused my reputation is none other than a certain kind of wisdom. What kind of wisdom? Human wisdom, perhaps(1). ” He proceeds to tell the story of his friend Chaerophon, who once asked the Oracle at Delphi whether there was anyone wiser than Socrates. The Oracle answered that there was not. Socrates did not agree and thought that he would try to prove the Oracle wrong. And so he set about seeking out Athenians with a reputation for wisdom in various regards in order to test their claims to knowledge through questioning. He discovered many with false claims to knowledge and none with genuine wisdom and ultimately concluded that he was the wisest. He reached this conclusion not because of any special knowledge he possessed that others did not, but rather because he recognized his own lack of knowledge and strived to learn more, while others thought that they were kno- edgeable but were not. Socrates’ conclusion that there is wisdom in recognizing the limitations of accepted knowledge represents the motivation for this book.
From reviews of the First Edition: "Being a concise introduction to the principles of neruopathology is a goal this book accomplishes admirably." Annals of Neurology; "unquestionably valuable as a reference text" Arch Path Lab Med; "a fine treatise which truly reflects the current knowledge of the discipline with a strong emphasis on morphologic aspects" Brain Pathology; "an excellent current reference work on neuropathology for practitioners in the various clinical and basic neurosciences" Journal of Neuropathology and Experimental Neurology.
In this book, Professor Victor Dubowitz manages to bridge the gap between clinical syndromes/disorders and their underlying pathologies. An internationally renowned figure in the field of muscle disease, Professor Dubowitz skillfully guides you through the complexities of pathologic diagnoses and their implications for clinical treatment. This reference describes the techniques of obtaining a muscle biopsy and examines the histochemical, histological, electron microscopical and molecular appearance of normal muscle and the pathology of individual muscle disease. Covers the entire range of diagnostic/investigative techniques, providing you with all of the necessary tools to formulate an accurate diagnosis. Offers a combination of clinical and pathological experience for a unique perspective on a complex and difficult area of diagnosis. Addresses the "knowledge explosion" over the last 10 years in the fields of molecular genetics and immunocytochemistry, including all of the latest clinically relevant investigative techniques. Focuses on diagnostic techniques and on the diagnostic interpretation of results, giving you a portable reference book and a bench book in a single volume. Presents all the latest techniques in molecular diagnosis and immunocytochemistry as they apply to individual disorders. Includes high-quality, full-color illustrations throughout the book. Incorporates tables, summary charts, and boxes to provide a user-friendly, accessible format.
Comprehensive technical guide to performing biopsies and interpreting results using specialized techniques.
Nine years have elapsed since the second edition of this book was published. In this time the principal advances in neuromuscular diseases have been in the application of molecular genetics to understanding the aetiology and pathogenesis of this group of disorders. As a result many previously unrecognised disorders have been charac terised. Some clinical syndromes, such as the limb girdle dystrophies, have become better defined. In many such instances the new genetic information has led to major advances in knowledge of the biology of cell structures, for example, the membrane structural and channel proteins. The clinical syndromes themselves, and their patho logical and electrophysiological characteristics, however, remain as important as ever, since they constitute the clinical problem itself and, indeed, the database from which all other concepts emerge. Knowledge of the pathogenesis, genetics, and molecular biology of neuromuscular disorders is essential both in developing and applying new therapies and preventive measures, and in formulating genetic and prognostic advice. However, this informa tion does not necessarily always define clinically useful syndromes. Myotonia, for example, is an electrophysiological finding in some syndromes in which it is un detectable by clinical examination, although the phenomenon itself was originally defined as a clinical entity. The limb girdle muscular dystrophy syndromes can be defined by severity, distribution of weakness, age of onset, sex distribution and other characteristics and many of these can be better understood by study of the under lying defect in cell structural proteins.
Now in its 7th edition, this popular, must-have text remains the only encyclopedic resource for veterinary internal medical problems. The internationally acclaimed "gold standard" offers unparalleled coverage of pathophysiology, diagnosis, and treatment of diseases affecting dogs and cats, as well as the latest information on the genome, clinical genomics, euthanasia, innocent heart murmurs, hyperbaric medicine, home prepared and raw diets, obesity, botulism, artificial pacing of the heart, cancer vaccines, and more. The 7th edition combines the convenience of a two-volume printed textbook with the enhanced functionality of an Expert Consult website that enables you to electronically search your entire book and study more efficiently. With instant access to the most reliable information available, you’ll always be at the forefront of veterinary care! Fully searchable online text provides fast, easy access to the most reliable information in the field. More than 150 clinical algorithms throughout the text aid in disease-identification and decision-making. Expanded online chapter content enhances your understanding through additional text, illustrations, tables, and boxes. Hyperlinked client information sheets streamline reference of specific conditions and enhance communication with clients. Extensive online reference list directs you to full-text PubMed abstracts for additional research. Thoroughly updated and expanded content, including 90 new chapters, addresses the latest developments across the full spectrum of small animal care. Companion Expert Consult website enhances your learning experience with the ability to search the entire electronic text instantly for easy reference. Expert Consult also gives you instant access to: More than 150 procedural videos that guide you step-by-step through essential procedures. Audio files that help you identify heart abnormalities by their sound.
|Author||: John L. Pfenninger,Grant C. Fowler|
|Publisher||: Elsevier Health Sciences|
|Release Date||: 2010-09-23|
|ISBN 10||: 1455700924|
|Pages||: 1776 pages|
Pfenninger and Fowler’s Procedures for Primary Care, 3rd Edition is a comprehensive, "how-to" resource offering step-by-step strategies for nearly every medical procedure that can be performed in an office, hospital, or emergency care facility by primary care clinicians. . Designed for everyday practice, the outline format allows speedy reference while the detailed text and clear illustrations guide you through each procedure. The new edition of this best-selling book features full-color illustrations and easy access to the complete contents and illustrations, patient forms, and more online at www.expertconsult.com. Understand how to proceed, step by step, thanks to detailed text and illustrations. Locate critical information at a glance with numerous boxes and tables. Use the book for years with minimal wear and tear thanks to its sturdy cover. Patient education handouts to educate, save time, and reduce liability Coding guidelines included This best selling text now includes full color photos and new sections on Aesthetic and Hospitalist Procedures in addition to an update of all the previous procedures discussed in prior editions! Access the complete contents and illustrations online, download patient education handouts and consent forms, view lists of device manufacturers, and more at www.expertconsult.com. Offer your patients a variety of cosmetic procedures using lasers and pulsed-light devices (including individual chapters on procedures for hair removal, photorejuvenation, , skin tightening and skin resurfacing, and tattoo removal), botulinum toxin, as well as new coverage of cosmeceutical skin care, tissue fillers, and photodynamic therapy. Master new procedures such as maggot treatment for chronic ulcers, endovenous vein closure, stress echo, insertion of the contraceptive capsule (Implanon) and tubal implant (Essure), musculoskeletal ultrasound, no-needle/no-scalpel vasectomy, procedures to treat acute headaches, and more. Don’t worry! All the more basic office procedures are still included...with improved and updated discussions! Pfenninger and Fowler provide the latest and most comprehensive information on medical procedures that allow primary care physicians to more effectively treat their patients.
|Author||: Nanette C. Joyce,Craig M. McDonald|
|Publisher||: Elsevier Health Sciences|
|Release Date||: 2012-09-16|
|ISBN 10||: 145574784X|
|Pages||: 240 pages|
Neuromuscular disease is a broad term that encompasses many diseases and ailments that either directly or indirectly impair the function of the body’s muscle system, via the nerves. This issue of PMR will provide an overview of current treatments and therapies for a variety of diseases. The GEs have gone through every issue published since 1998, and these 23 chapters will be meant to fill the numerous gaps in PMR’s coverage of the field over the past decade. The issue will include chapters on different treatment techniques, such as exercises, stretches, and nutrition. It will also provide chapters focusing on specific areas of the body, specific conditions, and an update on mobility technology for those with NMDs.
Important strides have been made in understanding the pathophysiologic basis of many inflammatory conditions in recent years, but rheumatology remains a discipline in which diagnosis is rooted in the medical history skillfully extracted from the patient, the careful physical examination, and the discriminating use of laboratory tests and imaging. Moreover, selection of the most appropriate therapy for patients with rheumatic diseases also remains heavily reliant upon clinical experience. Medical disciplines such as rheumatology that depend significantly upon clinical wisdom are prone to the development of systems of ‘Pearls’ and ‘Myths,’ related to the diseases they call their own, a ‘Pearl’ being a nugget of truth about the diagnosis or treatment of a particular disease that has been gained by dint of clinical experience and a ‘Myth’ being a commonly held belief that influences the practice of many clinicians – but is false. This book will pool together the clinical wisdom of seasoned, expert rheumatologists who participate in the care of patients with autoimmune diseases, systemic inflammatory disorders, and all other rheumatic conditions.
Campbell's Operative Orthopaedics, by Drs. S. Terry Canale and James H. Beaty, continues to define your specialty, guiding you through when and how to perform every state-of-the-art procedure that's worth using. With hundreds of new procedures, over 7,000 new illustrations, a vastly expanded video collection, and new evidence-based criteria throughout, it takes excellence to a new level...because that is what your practice is all about. Consult this title on your favorite e-reader with intuitive search tools and adjustable font sizes. Elsevier eBooks provide instant portable access to your entire library, no matter what device you're using or where you're located. Achieve optimal outcomes with step-by-step guidance on today's full range of procedures from Campbell’s Operative Orthopaedics - the most trusted and widely used resource in orthopedic surgery - authored by Drs. S. Terry Canale, James H. Beaty, and 42 other authorities from the world-renowned Campbell Clinic. Access the complete contents online with regular updates, view all the videos, and download all the illustrations at www.expertconsult.com. See how to proceed better than ever before with 45 surgical videos demonstrating hip revision, patellar tendon allograft preparation, open reduction internal fixation clavicle fracture, total shoulder arthroplasty, total elbow arthroplasty, and more - plus over 7,000 completely new step-by-step illustrations and photos commissioned especially for this edition. Make informed clinical choices for each patient, from diagnosis and treatment selection through post-treatment strategies and management of complications, with new evidence-based criteria throughout. Utilize the very latest approaches in hip surgery including hip resurfacing, hip preservation surgery, and treatment of hip pain in the young adult; and get the latest information on metal-on-metal hips so you can better manage patients with these devices. Improve your total joint arthroplasty outcomes by reviewing the long-term data for each procedure; and consider the pros and cons of new developments in joint implant technology, including "customized" implants and their effect on patient outcomes. Implement new practices for efficient patient management so you can accommodate the increasing need for high-quality orthopaedic care in our aging population.
This is one of the most prestigious and comprehensive texts on arthritis and related diseases, including osteoarthritis, rheumatoid arthritis, osteoporosis, lupus and more than one hundred others. It offers medical students and physicians a concise description of the current science, diagnosis, clinical consequences, and principles of management. New and expanded chapters heighten the translational nature of this edition. Students, trainees, and practicing clinicians all need a standard textbook that can change with the times and reflect recent strides taken in understanding and treating rheumatic disease. The Primer fills that need.
There are many tomes available detailing the histopathological features of both general surgical pathology entities and organ specific pathologies. In addition, several texts present aspects of developmental, fetal and placental pathology. However, for the specialist paediatric pathologist with a significant paediatric surgical specimen workload, and for the practising general surgical pathologist faced with reporting paediatric surgical specimens, although information regarding many of the specific entities affecting childhood is available in the former texts, such information requires searching of many disjointed books. Therefore, the aim of this publication is to present a comprehensive and detailed account, which brings together and covers all, or at least most, paediatric surgical pathological entities in a single volume, to allow rapid access for day-to-day use by practising histopathologists. The focus is on practical diagnostic issues throughout. Concentrates on the diagnostic and management issues that are relevant to the surgical pathologist when signing out a report: ie variant histopathological appearances, what the surgeon needs to know about grading. Comprehensive coverage of all entities that occur in children and adolescents.Provides the general surgical pathologist more used to interpreting adult specimens with a complete guide to all of the histopathologic differences in appearance compared to similar diseases in adults. Superb, high quality full color illustrations of key aspects of various diseases (histopathologic, molecular and clinical features) throughout.Provides a complete visual guide to paediatric neoplastic and non-neoplastic lesions will allow rapid identification of tumor or tumor-like lesions. Includes the latest techniques in immunohistochemistry and molecular genetics in an integrated manner.Provides comprehensive information on all of the investigative contexts relevant to formulating an accurate. diagnosis. Information organized simply and consistently throughout in bullet point format.Helps the users find what they’re looking for quickly and easily. Extensive use of tables to list all of the possible differential diagnosis of each tumor and tumor-like entity. User-friendly summary of key points to be considered in problem areas presented in a way that makes it ideal for a busy surgical pathologist.
|Author||: Antoine E. El-Khoury|
|Publisher||: CRC Press|
|Release Date||: 1999-03-31|
|ISBN 10||: 9780849396120|
|Pages||: 280 pages|
Containing all the new as well as classical methodologies used in the investigation of amino acid and protein metabolism in human and animal models, this book is needed because of the dramatic increase in research in this field. There is no other book currently on the market that covers these methods of investigation. Methods for Investigation of Amino Acid and Protein Metabolism explores areas such as amino acid transfer across tissue membranes, past and new applications using stable isotopes, protein synthesis in organs and tissues, and more. Because of the importance of research methods in the field of amino acid and protein nutrition and metabolism, this book facilitates the reader's integration of the concepts involved in these investigative research methods and their corollaries. In addition to helping any nutrition investigator design and conduct appropriate research protocols in this area of nutrition, this book assists students who are planning to investigate amino acid and protein metabolism in humans or laboratory animals.
Physical Medicine and Rehabilitation presents today’s best physiatry knowledge and techniques, ideal for the whole rehabilitation team. This trusted reference delivers the proven science and comprehensive guidance you need to offer every patient maximum pain relief and optimal return to function. In this new edition, Dr. Randall L. Braddom covers current developments in interventional injection procedures, the management of chronic pain, integrative medicine, recent changes in the focus of stroke and brain injury rehabilitation, and much more. Access the complete contents online along with 1000 self-assessment questions at www.expertconsult.com. Gain a clear visual understanding of important concepts thanks to 1400 detailed illustrations—1000 in full color. Find and apply the information you need easily with each chapter carefully edited by Dr. Braddom and his associates for consistency, succinctness, and readability. Access the fully searchable text online at Expert Consult, as well as 1000 self-assessment questions. Master axial and peripheral joint injections through in-depth coverage of the indications for and limitations of these therapies. Make optimal use of ultrasound in diagnosis and treatment. Get a broader perspective on your field from a new chapter on PM&R in the international community.