Laboratory Exercises in Molecular Pathology is organized by major organ systems and then by disease type. In each exercise, there is a distillation of essential disease-specific information (related to frequency, risk factors, disease manifestations) and a description of disease pathogenesis (molecular and cellular) that is based upon accepted principles from the literature. Further, each exercise is illustrated with (1) gross specimens, (2) microscopic histopathology, (3) immunohistochemistry and/or in situ hybridization (when applicable), (4) laboratory techniques to probe the molecular nature of the pathological lesions, and (5) molecular diagnostics (when applicable). In addition, each exercise contains one or more cases studies to provide students with exposure to the clinical workup of a patient, based upon physical examination findings, traditional pathological analysis, and state-of-the-art molecular assessment. The laboratory techniques included emphasize the dissection of molecular and cellular pathogenesis of the disease in question. Hence, students will see examples of laboratory results that illustrate how pathways were elucidated. Further, the sections on molecular diagnostics illustrate how molecular testing exploits what is known about molecular pathogenesis in a specific disease setting. The manual does not present molecular methods and techniques in isolation; it illustrates how these experimental approaches and applied methodologies can be used to generate meaningful information in the clinical setting. Presents foundational information, examples of gross and microscopic pathology, and examples of molecular approaches and molecular testing all in one resource Offers an ancillary website with videos corresponding to the evaluation of gross specimens, the assessment of microscopic images, and the demonstration of how laboratory techniques are performed Features exercises which contain one or more cases studies to provide students with exposure to the clinical workup of a patient, based upon physical examination findings, traditional pathological analysis, and state-of-the-art molecular assessment Provides case studies which include self-assessment modules and decision trees that allow students to make mistakes and then receive explanations
Continuing in the tradition of its predecessors, this new edition combines an informal, easy to read style with a thorough introduction to concepts and terminology of plant pathology. After reviewing fundamental concepts, the book discusses groups of plant pathogens and molecular tools for studying them, pathogen interactions, epidemiology and disease control, and special topics in plant pathology. The book details various disease-causing organisms, including viruses, fungi, prokaryotics, nematodes, and various biotic agents. It also examines various plant-pathogen interactions, molecular attack strategies, extracellular enzymes, host defenses, and disruption of plant function. New in the Third Edition Molecular plant-fungal interactions Expanded treatment of molecular tools Advanced biocontrol concepts How to use and care for microscopes
Diagnostic Molecular Pathology: A Guide to Applied Molecular Testing is organized around disease types (genetic disease, infectious disease, neoplastic disease, among others). In each section, the authors provide background on disease mechanisms and describe how laboratory testing is built on knowledge of these mechanisms. Sections are dedicated to general methodologies employed in testing (to convey the concepts reflected in the methods), and specific description of how these methods can be applied and are applied to specific diseases are described. The book does not present molecular methods in isolation, but considers how other evidence (symptoms, radiology or other imaging, or other clinical tests) is used to guide the selection of molecular tests or how these other data are used in conjunction with molecular tests to make diagnoses (or otherwise contribute to clinical workup). In addition, final chapters look to the future (new technologies, new approaches) of applied molecular pathology and how discovery-based research will yield new and useful biomarkers and tests. Diagnostic Molecular Pathology: A Guide to Applied Molecular Testing contains exercises to test readers on their understanding of how molecular diagnostic tests are utilized and the value of the information that can be obtained in the context of the patient workup. Readers are directed to an ancillary website that contains supplementary materials in the form of exercises where decision trees can be employed to simulate actual clinical decisions. Focuses on the menu of molecular diagnostic tests available in modern molecular pathology or clinical laboratories that can be applied to disease detection, diagnosis, and classification in the clinical workup of a patient Explains how molecular tests are utilized to guide the treatment of patients in personalized medicine (guided therapies) and for prognostication of disease Features an ancillary website with self-testing exercises where decision trees can be employed to simulate actual clinical decisions Highlights new technologies and approaches of applied molecular pathology and how discovery-based research will yield new and useful biomarkers and tests
Molecular Diagnostics, Third Edition, focuses on the technologies and applications that professionals need to work in, develop, and manage a clinical diagnostic laboratory. Each chapter contains an expert introduction to each subject that is next to technical details and many applications for molecular genetic testing that can be found in comprehensive reference lists at the end of each chapter. Contents are divided into three parts, technologies, application of those technologies, and related issues. The first part is dedicated to the battery of the most widely used molecular pathology techniques. New chapters have been added, including the various new technologies involved in next-generation sequencing (mutation detection, gene expression, etc.), mass spectrometry, and protein-specific methodologies. All revised chapters have been completely updated, to include not only technology innovations, but also novel diagnostic applications. As with previous editions, each of the chapters in this section includes a brief description of the technique followed by examples from the area of expertise from the selected contributor. The second part of the book attempts to integrate previously analyzed technologies into the different aspects of molecular diagnostics, such as identification of genetically modified organisms, stem cells, pharmacogenomics, modern forensic science, molecular microbiology, and genetic diagnosis. Part three focuses on various everyday issues in a diagnostic laboratory, from genetic counseling and related ethical and psychological issues, to safety and quality management. Presents a comprehensive account of all new technologies and applications used in clinical diagnostic laboratories Explores a wide range of molecular-based tests that are available to assess DNA variation and changes in gene expression Offers clear translational presentations by the top molecular pathologists, clinical chemists, and molecular geneticists in the field
|Author||: Robert N. Trigiano,Mark Townsend Windham,Alan S. Windham|
|Publisher||: CRC Press|
|Release Date||: 2008|
|Pages||: 558 pages|
Including contributions from specialists with extensive experience in the field, this second edition features new chapters on pathogenic prokaryotes, soilborne pathogens, and host-pathogen interactions. It increases coverage of a number of molecular techniques-including real-time PCR methods for pathogen detection, AFLPs, and AGDIA methods-and insect vectoring of bacterial pathogens with potential control strategies. A more detailed discussion of disease diagnosis is also provided. An accompanying CD-ROM offers a wide range of full-colour illustrations to enhance understanding of concepts. Additional references and case studies have been added to the text.
|Author||: Robert N. Trigiano|
|Publisher||: CRC Press|
|Release Date||: 2007-11-30|
|ISBN 10||: 1482226936|
|Pages||: 576 pages|
Revised and updated with new concepts, case studies, and laboratory exercises, Plant Pathology Concepts and Laboratory Exercises, Second Edition supplies highly detailed and accurate information in a well-organized and accessible format. New additions to the second edition include five new topic and exercise chapters on soilborne pathogens, molecular tools, biocontrol, and plant-fungal interactions, information on in vitro pathology, an appendix on plant pathology careers, and how to use and care for the microscope. An accompanying cd-rom contains figures from the text as well as supplemental full-color photos and PowerPoint slides. Unique Learning Tools Retaining the informal style of the previous edition, this volume begins each topic with a concept box to highlight important ideas. Several laboratory exercises support each topic and cater to a wide range of skill sets from basic to complex. Procedure boxes for the experimental exercises give detailed outlines and comments on the experiments, step by step instruction, anticipated results, and thought provoking questions. Case studies of specific diseases and processes are presented as a bulleted list supplying essential information at a glance. Comprehensive Coverage Divided into six primary parts, this valuable reference introduces basic concepts of plant pathology with historical perspectives, fundamental ideas of disease, and disease relationships with the environment. It details various disease-causing organisms including viruses, prokaryotic organisms, plant parasitic nematodes, fungi, plant parasitic seed plants, and other biotic and abiotic diseases. Exploring various plant-pathogen interactions including treatments of molecular attack strategies, extracellular enzymes, host defenses, and disruption of plant function, the book presents the basic ideas of epidemiology, control strategies, and disease diagnosis.
As the molecular basis of human disease becomes better characterized, and the implications for understanding the molecular basis of disease becomes realized through improved diagnostics and treatment, Molecular Pathology, Second Edition stands out as the most comprehensive textbook where molecular mechanisms represent the focus. It is uniquely concerned with the molecular basis of major human diseases and disease processes, presented in the context of traditional pathology, with implications for translational molecular medicine. The Second Edition of Molecular Pathology has been thoroughly updated to reflect seven years of exponential changes in the fields of genetics, molecular, and cell biology which molecular pathology translates in the practice of molecular medicine. The textbook is intended to serve as a multi-use textbook that would be appropriate as a classroom teaching tool for biomedical graduate students, medical students, allied health students, and others (such as advanced undergraduates). Further, this textbook will be valuable for pathology residents and other postdoctoral fellows that desire to advance their understanding of molecular mechanisms of disease beyond what they learned in medical/graduate school. In addition, this textbook is useful as a reference book for practicing basic scientists and physician scientists that perform disease-related basic science and translational research, who require a ready information resource on the molecular basis of various human diseases and disease states. Explores the principles and practice of molecular pathology: molecular pathogenesis, molecular mechanisms of disease, and how the molecular pathogenesis of disease parallels the evolution of the disease Explains the practice of “molecular medicine and the translational aspects of molecular pathology Teaches from the perspective of “integrative systems biology Enhanced digital version included with purchase
Studies of the interactions between plants and their viral, bacterial and fungal pathogens are of major importance in plant and crop production. More than 10% of potential agricultural yield is lost to these organisms annually worldwide, and major epidemics can cause significant local economic and environmental damage. Molecular Plant Pathology addresses the underlying molecular principles of plant/pathogen interactions, in a readily-accessible textbook format.
|Author||: Bonnie H. Ownley,Robert N. Trigiano|
|Publisher||: CRC Press|
|Release Date||: 2016-03-26|
|ISBN 10||: 9781466500815|
|Pages||: 450 pages|
Continuing in the tradition of its predecessors, this new edition combines an informal, easy to read style with a thorough introduction to concepts and terminology of plant pathology. After reviewing fundamental concepts, the book discusses groups of plant pathogens and molecular tools for studying them, pathogen interactions, epidemiology and disease control, and special topics in plant pathology. The book details various disease-causing organisms, including viruses, fungi, prokaryotics, nematodes, and various biotic agents. It also examines various plant-pathogen interactions, molecular attack strategies, extracellular enzymes, host defenses, and disruption of plant function.
Plant Pathology explores the topic of plant pathology and aligns classic studies and knowledge in the topic with the current state of research, in an accessible format. The text is supported by summary tables of key information and, where appropriate, schematic diagrams to reinforce difficult concepts such as the process of disease infection, cell-to-cell recognition, and plant breeding mechanisms used to develop resistant cultivars. The compendium of diseases focuses on important and major economic disease organisms from a number of crop and ornamental plants, including a dedicated section on fruit crops. The compendium is supported by original photographs, photomicrographs and electron micrographs of key pathogens and the development of structures such as the haustoria and the hypha, and show processes of cellular degradation. The section on applied disease management contains short case studies highlighting key disease organisms affecting the crops of a range of growers, illustrating the environment, disease symptoms and control strategies these growers are currently using to mitigate loss of production.