introduction to electrophysiological methods and instrumentation

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Introduction to Electrophysiological Methods and Instrumentation
Author : Franklin Bretschneider,Jan R. de Weille
Publisher : Academic Press
Release Date : 2018-11-13
ISBN 10 : 0128142111
Pages : 370 pages
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Introduction to Electrophysiological Methods and Instrumentation, Second Edition covers all topics of interest to electrophysiologists, neuroscientists and neurophysiologists, from the reliable penetration of cells and the behavior and function of the equipment, to the mathematical tools available for analyzing data. It discusses the pros and cons of techniques and methods used in electrophysiology and how to avoid pitfalls. Although the basics of electrophysiological techniques remain the principal purpose of this second edition, it now integrates several current developments, including, amongst others, automated recording for high throughput screening and multimodal recordings to correlate electrical activity with other physiological parameters collected by optical means. This book provides the electrophysiologist with the tools needed to understand his or her equipment and how to acquire and analyze low-voltage biological signals. Introduces possibilities and solutions, along with the problems, pitfalls, and artefacts of equipment and electrodes Discusses the particulars of recording from brain tissue slices, oocytes and planar bilayers Describes optical methods pertinent to electrophysiological practice Presents the fundamentals of signal processing of analogue signals, spike trains and single channel recordings, along with procedures for signal recording and processing Includes appendices on electrical safety and foundations of useful mathematical tools

Electrophysiological Methods
Author : William L. Nastuk
Publisher : Academic Press
Release Date : 2017-01-31
ISBN 10 : 1483191796
Pages : 478 pages
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Electrophysiological Methods presents a compilation of essays relating to the field of electricity. One topic is the introduction and analysis of electrical stimulation. Other types of stimulation are thermal stimulation, light stimulation, touch stimulation, and auditory stimulation. Microelectrodes act as links by which small regions in biological tissues are electrically joined to amplifying and recording devices. This topic is explored more thoroughly in the book. The physiological activity of cells in nerves, muscles, secretory organs, and other biological structures is accompanied by electrical changes which appear across the cell membrane. This occurrence is another topic in the text. Intracellular techniques employed to record transmembrane potentials and to stimulate cells are likewise presented. A section of the book presents microelectrophoresis which is a method wherein electrical flow is employed to manipulate the ejection of chemical substances from fine microelectrodes or micropipettes. The book will provide useful information to neurologist, cytologist, doctors, students, and researchers in the field of medicine.

Patch Clamping
Author : Areles Molleman
Publisher : John Wiley & Sons
Release Date : 2003-06-13
ISBN 10 : 0470856513
Pages : 186 pages
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Patch clamping is a widely applied electrophysiological technique for the study of ion channels; membrane proteins that regulate the flow of ions across cellular membranes and therefore influence the physiology of all cells. Patch Clamping aims to cover the basic principles and practical applications of this important technique. Starting with a review of the history of patch clamping, the text then goes on to cover the basic principles, platforms, equipment and environmental control, and will also include coverage of preparation types, recording modes and analysis of results. This book will explain the basic principles and practical application of patch clamp electrophysiology Written in a non-technical style to ensure its broad appeal to novice users Takes a practical approach This self-contained guide provides everything a practising patch clamp electrophysiologist needs to know to master this technique, including an overview of membrane biophysics, standard experimental design, data analysis, and technical concerns

Basic Electrophysiological Methods
Author : Ellen Covey,Matt Carter
Publisher : Oxford University Press, USA
Release Date : 2015-03-25
ISBN 10 : 0199939802
Pages : 240 pages
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Basic Electrophysiological Methods provides a concise and easy-to-read guide on a selection of the most important contemporary electrophysiological techniques, their implementation, applications, and ways in which they can be combined and integrated with neuroscientific techniques. Intended for students, postdocs, and faculty with a basic neuroscience background, this text will not obscure the relevant technical details with textbook neuroscience tutorials as many other books do. Instead, each chapter provides a conscientious overview of the underlying theory -- a comprehensive description of equipment, materials, methods, data management, and analysis -- a troubleshooting guide, and a list of frequently asked questions. No book or online resource can function as strictly a DIY set of instructions on how to implement a complex technique. However, this book provides a fundamental and accessible set of information intended to form a foundation prior to, during, and after hands-on experience and training, greatly facilitating the initial learning process and subsequent fine-tuning of technical details.

Basic Electrophysiological Methods
Author : Ellen Covey,Matt Carter
Publisher : Oxford University Press
Release Date : 2015-02-25
ISBN 10 : 0199939861
Pages : 256 pages
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Basic Electrophysiological Methods provides a concise and easy-to-read guide on a selection of the most important contemporary electrophysiological techniques, their implementation, applications, and ways in which they can be combined and integrated with neuroscientific techniques. Intended for students, postdocs, and faculty with a basic neuroscience background, this text will not obscure the relevant technical details with textbook neuroscience tutorials as many other books do. Instead, each chapter provides a conscientious overview of the underlying theory -- a comprehensive description of equipment, materials, methods, data management, and analysis -- a troubleshooting guide, and a list of frequently asked questions. No book or online resource can function as strictly a DIY set of instructions on how to implement a complex technique. However, this book provides a fundamental and accessible set of information intended to form a foundation prior to, during, and after hands-on experience and training, greatly facilitating the initial learning process and subsequent fine-tuning of technical details.

Introduction to Experimental Biophysics
Author : Jay L. Nadeau
Publisher : CRC Press
Release Date : 2016-04-19
ISBN 10 : 1439897409
Pages : 672 pages
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Increasing numbers of physicists, chemists, and mathematicians are moving into biology, reading literature across disciplines, and mastering novel biochemical concepts. To succeed in this transition, researchers must understand on a practical level what is experimentally feasible. The number of experimental techniques in biology is vast and often s

Instrumentation and Techniques for Measuring the Quality of Insect Vision with the Electroretinogram
Author : Herndon R. Agee
Publisher : N.A
Release Date : 1977
ISBN 10 :
Pages : 13 pages
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MEG
Author : Peter Hansen,Morten Kringelbach,Riitta Salmelin
Publisher : Oxford University Press
Release Date : 2010-07-01
ISBN 10 : 9780199719136
Pages : 448 pages
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Magnetoencephalography (MEG) is an exciting brain imaging technology that allows real-time tracking of neural activity, making it an invaluable tool for advancing our understanding of brain function. In this comprehensive introduction to MEG, Peter Hansen, Morten Kringelbach, and Riitta Salmelin have brought together the leading researchers to provide the basic tools for planning and executing MEG experiments, as well as analyzing and interpreting the resulting data. Chapters on the basics describe the fundamentals of MEG and its instrumentation, and provide guidelines for designing experiments and performing successful measurements. Chapters on data analysis present it in detail, from general concepts and assumptions to analysis of evoked responses and oscillatory background activity. Chapters on solutions propose potential solutions to the inverse problem using techniques such as minimum norm estimates, spatial filters and beamformers. Chapters on combinations elucidate how MEG can be used to complement other neuroimaging techniques. Chapters on applications provide practical examples of how to use MEG to study sensory processing and cognitive tasks, and how MEG can be used in a clinical setting. These chapters form a complete basic reference source for those interested in exploring or already using MEG that will hopefully inspire them to try to develop new, exciting approaches to designing and analyzing their own studies. This book will be a valuable resource for researchers from diverse fields, including neuroimaging, cognitive neuroscience, medical imaging, computer modelling, as well as for clinical practitioners.

Plant Electrophysiology
Author : Alexander G. Volkov
Publisher : Springer Science & Business Media
Release Date : 2007-04-19
ISBN 10 : 354037843X
Pages : 508 pages
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This book compiles new findings in plant electrophysiology from the work of internationally renowned experts in the fields of electrophysiology, bio-electrochemistry, biophysics, signal transduction, phloem transport, tropisms, ion channels, plant electrochemistry, and membrane transport. Opening with a historical introduction, the book reviews methods in plant electrophysiology, introducing such topics as measuring membrane potentials and ion fluxes, path-clamp technique, and electrochemical sensors. The coverage includes experimental results and their theoretical interpretation.

Physical Techniques in Biological Research: Electrophysiological methods, pts. A-B
Author : Dan Houston Moore,Arthur Wagg Pollister
Publisher : N.A
Release Date : 1966
ISBN 10 :
Pages : 329 pages
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Physical Techniques in Biological Research: Electrophysiological methods. pts. A-B
Author : Gerald Oster,Arthur Wagg Pollister,William L. Nastuk
Publisher : N.A
Release Date : 1955
ISBN 10 :
Pages : 329 pages
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Electrochemical Methods for Neuroscience
Author : Adrian C. Michael,Laura Borland
Publisher : CRC Press
Release Date : 2006-12-13
ISBN 10 : 1420005863
Pages : 544 pages
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Since the first implant of a carbon microelectrode in a rat 35 years ago, there have been substantial advances in the sensitivity, selectivity and temporal resolution of electrochemical techniques. Today, these methods provide neurochemical information that is not accessible by other means. The growing recognition of the versatility of electrochemical techniques indicates a need for a greater understanding of the scientific foundation and use of these powerful tools. Electrochemical Methods for Neuroscience provides an updated summary of the current, albeit evolving, state of the art and lays the scientific foundation for incorporating electrochemical techniques into on-going or newly emerging research programs in the neuroscience disciplines. With contributions from pioneers in the field, the text outlines the applications and benefits of a wide range of electrochemical techniques. It explores the methodology behind the acquisition of neurochemical and neurobiological data through continuous amperometry, fast scan cyclic voltammetry, high-speed chronoamperometry, ion-selective microelectrodes, enzyme based microelectrodes, and in vivo voltammetry with telemetry. The text also introduces emerging concepts in the field such as the correlation of electrochemical recordings with information obtained from patch clamp, electrophysiological, and behavioral techniques. By presenting up-to-date information on the growing collection of electrochemical methods, microsensors, and research techniques, Electrochemical Methods for Neuroscience assists seasoned researchers and newcomers to the field in making sound decisions about adopting the most appropriate of these tools for their future research objectives.

Electrical Properties of Cells
Author : Louis J. DeFelice
Publisher : Springer Science & Business Media
Release Date : 1997-07-31
ISBN 10 : 9780306453458
Pages : 244 pages
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DeFelice presents this intricate subject in an easy-to-follow, stepwise fashion: he reviews the fundamentals of electricity; transfers those principles to a biological context; and expands the discussion to encompass the subject's practical dimensions. Clear definitions and intuitive descriptions characterize the presentation, which is complemented by over 150 drawings and graphs. Mathematics is kept to the minimum necessary. The text covers both excitable and non-excitable membranes and includes the plasma membrane as well as intracellular membranes. A unique, `electronics-made-simple' appendix, designed specifically for biologists, treats the operational amplifiers used in patch clamp, and other appendices offer solutions to equations and examples that illustrate principles.

The Cognitive Electrophysiology of Mind and Brain
Author : Alberto Zani,Alice Proverbio
Publisher : Academic Press
Release Date : 2003
ISBN 10 : 9780127754215
Pages : 436 pages
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When his beloved donkey becomes ill, a young Italian boy is determined to take her to the crypt of St. Francis in Assisi in hopes of making her well.

In Vivo Optical Imaging of Brain Function, Second Edition
Author : Ron D. Frostig
Publisher : CRC Press
Release Date : 2009-05-06
ISBN 10 : 142007685X
Pages : 428 pages
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These are exciting times for the field of optical imaging of brain function. Rapid developments in theory and technology continue to considerably advance understanding of brain function. Reflecting changes in the field during the past five years, the second edition of In Vivo Optical Imaging of Brain Function describes state-of-the-art techniques and their applications for the growing field of functional imaging in the live brain using optical imaging techniques. New in the Second Edition: Voltage-sensitive dyes imaging in awake behaving animals Imaging based on genetically encoded probes Imaging of mitochondrial auto-fluorescence as a tool for cortical mapping Using pH-sensitive dyes for functional mapping Modulated imaging Calcium imaging of neuronal activity using 2-photon microscopy Fourier approach to optical imaging Fully updated chapters from the first edition Leading Authorities Explore the Latest Techniques Updated to reflect continuous development in this emerging research area, this new edition, as with the original, reaches across disciplines to review a variety of non-invasive optical techniques used to study activity in the living brain. Leading authorities from such diverse areas as biophysics, neuroscience, and cognitive science present a host of perspectives that range from a single neuron to large assemblies of millions of neurons, captured at various temporal and spatial resolutions. Introducing techniques that were not available just a few years ago, the authors describe the theory, setup, analytical methods, and examples that highlight the advantages of each particular method.

Measurement, Instrumentation, and Sensors Handbook
Author : John G. Webster,Halit Eren
Publisher : CRC Press
Release Date : 2018-09-03
ISBN 10 : 1351832999
Pages : 3559 pages
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This new edition of the bestselling Measurement, Instrumentation, and Sensors Handbook brings together all aspects of the design and implementation of measurement, instrumentation, and sensors. Reflecting the current state of the art, it describes the use of instruments and techniques for performing practical measurements in engineering, physics, chemistry, and the life sciences; explains sensors and the associated hardware and software; and discusses processing systems, automatic data acquisition, reduction and analysis, operation characteristics, accuracy, errors, calibrations, and the incorporation of standards for control purposes. Organized according to measurement problem, the Second Edition: Consists of 2 volumes Features contributions from 240+ field experts Contains 53 new chapters, plus updates to all 194 existing chapters Addresses different ways of making measurements for given variables Emphasizes modern intelligent instruments and techniques, human factors, modern display methods, instrument networks, and virtual instruments Explains modern wireless techniques, sensors, measurements, and applications A concise and useful reference for engineers, scientists, academic faculty, students, designers, managers, and industry professionals involved in instrumentation and measurement research and development, Measurement, Instrumentation, and Sensors Handbook, Second Edition provides readers with a greater understanding of advanced applications.

Engineered Nanopores for Bioanalytical Applications
Author : Joshua B. Edel,Tim Albrecht
Publisher : William Andrew
Release Date : 2013-03-19
ISBN 10 : 143773474X
Pages : 192 pages
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Engineered Nanopores for Bioanalytical Applications is the first book to focus primarily on practical analytical applications of nanopore development. These nanoscale analytical techniques have exciting potential because they can be used in applications such as DNA sequencing, DNA fragment sizing, DNA/protein binding, and protein/protein binding. This book provides a solid professional reference on nanopores for readers in academia, industry and engineering and biomedical fields. In addition, the book describes the instrumentation, fabrication, and experimental methods necessary to carry out nanopore-based experiments for developing new devices. Includes application case studies for detection, identification and analysis of biomolecules and related functional nanomaterials Introduces the techniques of manufacturing solid state materials with functional nanopores Explains the use of nanopores in DNA sequencing and the wider range of applications from environmental monitoring to forensics

Sensors, Nanoscience, Biomedical Engineering, and Instruments
Author : Richard C. Dorf
Publisher : CRC Press
Release Date : 2018-10-03
ISBN 10 : 142000316X
Pages : 392 pages
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In two editions spanning more than a decade, The Electrical Engineering Handbook stands as the definitive reference to the multidisciplinary field of electrical engineering. Our knowledge continues to grow, and so does the Handbook. For the third edition, it has expanded into a set of six books carefully focused on a specialized area or field of study. Each book represents a concise yet definitive collection of key concepts, models, and equations in its respective domain, thoughtfully gathered for convenient access. Sensors, Nanoscience, Biomedical Engineering, and Instruments provides thorough coverage of sensors, materials and nanoscience, instruments and measurements, and biomedical systems and devices, including all of the basic information required to thoroughly understand each area. It explores the emerging fields of sensors, nanotechnologies, and biological effects. Each article includes defining terms, references, and sources of further information. Encompassing the work of the world’s foremost experts in their respective specialties, Sensors, Nanoscience, Biomedical Engineering, and Instruments features the latest developments, the broadest scope of coverage, and new material on multisensor data fusion and MEMS and NEMS.

Experimental Methods and Instrumentation in Psychology
Author : Joseph B. Sidowski
Publisher : McGraw-Hill Companies
Release Date : 1966
ISBN 10 :
Pages : 803 pages
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Guide to Research Techniques in Neuroscience
Author : Matt Carter,Jennifer C. Shieh
Publisher : Academic Press
Release Date : 2009-09-25
ISBN 10 : 9780080951744
Pages : 408 pages
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Neuroscience is by definition a multidisciplinary field: some scientists study genes and proteins at the molecular level while others study neural circuitry. A single topic such as the auditory system can be studied using techniques from genetics, imaging, biochemistry, or electrophysiology. A young scientist must learn how to read the primary literature and then develop their own experiments. This book offers that scientist an overview of mainstream research techniques, provides guidelines on how to choose one technique over another, offers tips on analyzing data, and provides a list of references for additional detailed study. This book can also assist an experienced scientist understand published studies conducted outside their own subfield. *Written by Stanford University graduate students in neuroscience to provide a "hands-on" approach for other neuroscience graduate students *Techniques within one field will be compared so that user can select best technique for their experiment *Chapters include references (key articles, books, protocols) for additional detailed study *Data Analysis boxes in each chapter help with data interpretation and offer guidelines on how best to represent results *"Walk-through" boxes guide students through the experiment step-by-step