Electrophoresis is a widely used method in the field of life sciences, having multiple practical applications in physical, chemical, biochemical, and molecular biology domains. This book contains 8 chapters depicting various applications of this technique in biochemistry, molecular biology, and physical chemistry. This book presents the link between the exposed method and its applications in a very explicit manner and offers a wide range of practical examples. The book provides not only a vision of actual methods but also their necessary further improvements and suggested developments. Therefore, a particular attention was given to the described techniques as true guidelines in the fields where electrophoresis is recommended, being useful for not only the scientists but also the laboratory clinicians.
|Author||: Kiyokatsu Jinno|
|Release Date||: 1997-03-21|
|Pages||: 426 pages|
This book provides an in-depth understanding of molecular recognition mechanisms in designing chromatographical processes for separations. The title explains the importance of chemistry in chromatography and molecule-molecule interaction mechanisms and extends the concepts of separation to isomers and chiral isomers. "Chromatographic Separations Based on Molecular Recognition" discusses the theoretical interpretations of chromatographic retention processes, explains the molecular recognition concept for the separation of various isomers, and provides a foundation in separation science which will lead to a better grasp of other chemical fields and simplify the design and synthesis of novel stationary phases.
Antibody Engineering comprises in vitro selection and modification of human antibodies including humanization of mouse antibodies for therapy, diagnosis, and research. This book comprises an overview about the generation of antibody diversity and essential techniques in antibody engineering: construction of immune, naive and synthetic libraries, all available in vitro display methods, humanization by chain shuffling, affinity maturation techniques, de novo synthesis of antibody genes, colony assays for library screening, construction of scFvs from hybridomas, and purification of monoclonal antibodies by exclusion chromatography. In addition, other topics that are discussed in this book are application and mechanism of single domain antibodies, structural diversity of antibodies, immune-mediated skin reactions induced by TNF-alpha recombinant antibodies, and bioinformatic approaches to select pathogen-derived peptide sequences for antibody targets.
Drug Design and Development outlines the processes involved in the design and development of new drugs and emphasises the significance of these processes to the practice of pharmacy.
HPLC for Pharmaceutical Scientists is an excellent book for both novice and experienced pharmaceutical chemists who regularly use HPLC as an analytical tool to solve challenging problems in the pharmaceutical industry. It provides a unified approach to HPLC with an equal and balanced treatment of the theory and practice of HPLC in the pharmaceutical industry. In-depth discussion of retention processes, modern HPLC separation theory, properties of stationary phases and columns are well blended with the practical aspects of fast and effective method development and method validation. Practical and pragmatic approaches and actual examples of effective development of selective and rugged HPLC methods from a physico-chemical point of view are provided. This book elucidates the role of HPLC throughout the entire drug development process from drug candidate inception to marketed drug product and gives detailed specifics of HPLC application in each stage of drug development. The latest advancements and trends in hyphenated and specialized HPLC techniques (LC-MS, LC-NMR, Preparative HPLC, High temperature HPLC, high pressure liquid chromatography) are also discussed.
|Author||: Klara Valko|
|Release Date||: 2000-10-13|
|ISBN 10||: 9780080541099|
|Pages||: 612 pages|
Separation Methods in Drug Synthesis and Purification
This book presents a unified outlook on counter-current, ion size exclusion, supercritical fluids, high-performance thin layers, and gas and size exclusion chromatographic techniques used for the separation and purification of organic and inorganic analytes. It also describes a number of green techniques, green sample preparation methods and optimization of solvent consumption in the chromatographic analysis of organic and inorganic analytes. This book offers a valuable resource not only for learners, but also for more experienced chromatographers, conveying a deeper understanding of green chromatographic techniques, green solvents and preparation methods.
This new volume, Promising Drug Molecules of Natural Origin, explores potential beneficial drug substances derived from nature. It presents the general principles, characteristics, evaluation techniques, and applications involved in drug molecules from natural sources, such as plants and marine life. With chapters from renowned experts from around the world, the chapters in this volume address the challenges of standardization of herbal medicines, methods of characterization of natural medicines and phyto-constituents, and quality control methods for herbal medicines. Several chapters in the book focus on the evolution of phyto-constituents in cancer therapeutics, while others deal with applications for other diseases, such as diabetes and neuroinflammatory disorders. The volume also specifically reviews heterocyclic drugs from plants. This volume will be a valuable resource for faculty and advanced students in pharmaceutics as well as researchers, scientists, and industry professionals in medicine and drug development.
This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the challenges that scale-up of production poses to the investigator. Many of the chapters also discuss generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins as a means to enhance their solubility and simplify their purification on an individual scale or in high-throughput systems. This book also provides readers with chapters that describe not just the more commonly used methods, but also recently developed approaches such as proteomic/mass spectrometric techniques and Lectin-based affinity chromatography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Chromatography: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the field of protein chromatography.