This concise professional reference provides a fundamental framework for the design and operation of solid-state fermentation bioreactors, enabling researchers currently working at laboratory scale to scale up their processes. The authors survey bioreactor types in common use, and describe in depth how to plan a project, and model heat transfer phenomena. The book includes case studies, and a review of practical issues involved in bioreactor performance.
In this expert handbook both the topics and contributors are selected so as to provide an authoritative view of possible applications for this new technology. The result is an up-to-date survey of current challenges and opportunities in the design and operation of bioreactors for high-value products in the biomedical and chemical industries. Combining theory and practice, the authors explain such leading-edge technologies as single-use bioreactors, bioreactor simulators, and soft sensor monitoring, and discuss novel applications, such as stem cell production, process development, and multi-product reactors, using case studies from academia as well as from industry. A final section addresses the latest trends, including culture media design and systems biotechnology, which are expected to have an increasing impact on bioreactor design. With its focus on cutting-edge technologies and discussions of future developments, this handbook will remain an invaluable reference for many years to come.
For the first time in a single volume, the design, characterisation and operation of the bioreactor system in which the tissue is grown is detailed. Bioreactors for Tissue Engineering presents an overall picture of the current state of knowledge in the engineering of bioreactors for several tissue types (bone, cartilage, vascular), addresses the issue of mechanical conditioning of the tissue, and describes the use of techniques such as MRI for monitoring tissue growth. This unique volume is dedicated to the fundamentals and application of bioreactor technology to tissue engineering products. Not only will it appeal to graduate students and experienced researchers in tissue engineering and regenerative medicine, but also to tissue engineers and culture technologists, academic and industrial chemical engineers, biochemical engineers and cell biologists who wish to understand the criteria used to design and develop novel systems for tissue growth in vitro.
Dynamic Single-Use Bioreactors Used in Modern Liter- and m3- Scale Biotechnological Processes: Engineering Characteristics and Scaling Up, by Christian Löffelholz, Stephan C. Kaiser, Matthias Kraume, Regine Eibl , Dieter Eibl. Orbitally Shaken Single-Use Bioreactors, by Wolf Klöckner, Sylvia Diederichs, Jochen Büchs. Therapeutic Human Cells: Manufacture for Cell Therapy/Regenerative Medicine by Christian van den Bos, Robert Keefe, Carmen Schirmaier, Michael McCaman. Fast Single-Use VLP Vaccine Productions Based on Insect Cells and the Baculovirus Expression Vector System: Influenza as Case Study by Regine Eibl, Nina Steiger, Sabine Wellnitz, Tiago Vicente, Corinne John, Dieter Eibl. Microbial High Cell Density Fermentations in a Stirred Single-Use Bioreactor by Thomas Dreher, Bart Walcarius, Ute Husemann, Franziska Klingenberg, Christian Zahnow, Thorsten Adams, Davy de Wilde, Peter Casteels, Gerhard Greller. Quorus Bioreactor: A New Perfusion-Based Technology for Microbial Cultivation by Sheena J. Fraser, Christian Endres. Cultivation of Marine Microorganisms in Single-Use Systems by Friederike Hillig, Maciej Pilarek, Stefan Junne, Peter Neubauer. Flexible Biomanufacturing Processes that Address the Needs of the Future by Bernhard Diel, Christian Manzke, Thorsten Peuker. An Approach to Quality and Security of Supply for Single-Use Bioreactors by Magali Barbaroux, Susanne Gerighausen, Heiko Hackel. A Risk Analysis for Production Processes with Disposable Bioreactors by Tobias Merseburger, Ina Pahl, Daniel Müller, Markus Tanner.
Bioreactors: Animal Cell Culture Control for Bioprocess Engineering presents the design, fabrication, and control of a new type of bioreactor meant especially for animal cell line culture. The new bioreactor, called the "see-saw bioreactor," is ideal for the growth of cells with a sensitive membrane. The see-saw bioreactor derives its name from its principle of operation in which liquid columns in either limb of the reactor alternately go up and down. The working volume of the reactor is small, to within 15 L. However, it can easily be scaled up for large production in volume of cell mass in the drug and pharmaceutical industries. The authors describe the principle of operation of the see-saw bioreactor and how to automatically control the bioprocess. They discuss different control strategies as well as the thorough experimental research they conducted on this prototype bioreactor in which they applied a time delay control for yield maximization. To give you a complete understanding of the design and development of the see-saw bioreactor, the authors cover the mathematical model they use to describe the kinetics of fermentation, the genetic algorithms used for deriving the optimal time trajectories of the bioprocess variables, and the corresponding control inputs for maximizing the product yield. One chapter is devoted to the application of time delay control. Following a description of the bioreactor’s working setup in the laboratory, the authors sum up their investigation and define the future scope of work in terms of design, control, and software sensors.
The editors of this special volume would first like to thank all authors for their excellent contributions. We would also like to thank Prof. Dr. Thomas Scheper, Dr. Marion Hertel and Ulrike Kreusel for providing the opportunity to compose this volume and Springer for organizational and technical support. Tissue engineering represents one of the major emerging fields in modern b- technology; it combines different subjects ranging from biological and material sciences to engineering and clinical disciplines. The aim of tissue engineering is the development of therapeutic approaches to substitute diseased organs or tissues or improve their function. Therefore, three dimensional biocompatible materials are seeded with cells and cultivated in suitable systems to generate functional tissues. Many different aspects play a role in the formation of 3D tissue structures. In the first place the source of the used cells is of the utmost importance. To prevent tissue rejection or immune response, preferentially autologous cells are now used. In particular, stem cells from different sources are gaining exceptional importance as they can be differentiated into different tissues by using special media and supplements. In the field of biomaterials, numerous scaffold materials already exist but new composites are also being developed based on polymeric, natural or xenogenic sources. Moreover, a very important issue in tissue en- neering is the formation of tissues under well defined, controlled and reprod- ible conditions. Therefore, a substantial number of new bioreactors have been developed.
Bioreactors: Sustainable Design and Industrial Applications in Mitigation of GHG Emissions presents and compares the foundational concepts, state-of-the-art design and fabrication of bioreactors. Solidly based on theoretical fundamentals, the book examines various aspects of the commercially available bioreactors, such as construction and fabrication, design, modeling and simulation, development, operation, maintenance, management and target applications for biofuels production and bio-waste management. Emerging issues in commercial feasibility are explored, constraints and pathways for upscaling, and techno-economic assessment are also covered. This book provides researchers and engineers in the biofuels and waste management sectors a clear, at-a-glance understanding of the actual potential of different advanced bioreactors for their requirements. It is a must-have reference for better-informed decisions when selecting the appropriate technology models for sustainable systems development and commercialization.
Air pollution, a major concern at the end of the 20th century, still remains a significant problem to be solved today. Traditionally, industrial waste gases have primarily been treated through physical or chemical methods. The search for new, efficient, and cost-effective alternative technologies has led to the development and, more recently, the improvement of gas phase bioreactors. This book is the first single text to provide a complete, comprehensive picture of all major biological reactors suitable for solving air pollution problems. The text describes the main features and covers the major aspects, from microbiological to engineering, as well as economic aspects, of the different types of bioreactors. The book also presents an in-depth review of the subject, from fundamental bench-scale research to industrial field applications related to the operation of full-scale systems successfully treating polluted air in Europe and the United States. Material dedicated to more conventional non-biological technologies has also been included, to provide a complete overview of the different alternative treatment processes. Audience: The different chapters have been written by international experts, as a result of a fruitful collaboration between European and American scientists and engineers. The resulting text is a high quality, valuable reference tool for a variety of readers, including graduate and postgraduate students, researchers, professors, engineers, and those professionals who are interested in environmental engineering and, more specifically, in innovative air pollution control technologies.
|Author||: William L. Hochfeld|
|Publisher||: CRC Press|
|Release Date||: 2006-06-22|
|ISBN 10||: 1420021311|
|Pages||: 408 pages|
Containing authoritative and in-depth coverage, Producing Biomolecular Materials Using Fermenters, Bioreactors, and Biomolecular Synthesizers examines the bioproduction systems that support the controlled, automated, and quantity growth of proteins. The book discusses the substance, character, makeup, and quality of the basic materials used in the production and downstream processing of boimolecular materials: raw materials, reagents, intermediates, and consumables. Dr. Hochfield gets right to the point, explaining just what must be done and how to do it effectively, then providing the formula necessary for reaching the required value, allowing you to simply plug-in your data and make protein. However, if you actually do need the origin and derivation of any given formula, you can go right to the extensive reference section in the Appendix, find the formula you need in the exact form that you need it, without having to wade through numerous pages of extraneous material. This classic work presents unparalled, detailed, and cutting-edge information on bioprocessing systems. A working reference and formulary for producing recombinant, bioactive, or other exotic proteins, peptides, and nucleic acids to specification, the text provides coverage of the related technologies, coupled with the extensive biotechnology glossary, manufacturer’s directories, extensive references, important formulae, charts, illustrations, comprehensive index, emphasis on practical techniques, time-proven methods, and essential applications. These features combine with its ingenious, easy-to-use layout to make it the resource you will consult on a regular basis.
|Author||: Christoph Brepols|
|Publisher||: IWA Publishing|
|Release Date||: 2011|
|ISBN 10||: 1843393050|
|Pages||: 138 pages|
Provides hands-on information on many apsects of MBR technology such as process configuration, investment and operation costs based on case studies and also in comparison to data from convential activated sludge (CAS) treatment processes. Includes recent research findings from the Eftverband in Germany, one of the pioneers in the full scale application of this technology -- Back cover.
It is my privilege to contribute the foreword for this unique volume entitled: “Plant Tissue Culture Engineering,” edited by S. Dutta Gupta and Y. Ibaraki. While there have been a number of volumes published regarding the basic methods and applications of plant tissue and cell culture technologies, and even considerable attention provided to bioreactor design, relatively little attention has been afforded to the engineering principles that have emerged as critical contributions to the commercial applications of plant biotechnologies. This volume, “Plant Tissue Culture Engineering,” signals a turning point: the recognition that this specialized field of plant science must be integrated with engineering principles in order to develop efficient, cost effective, and large scale applications of these technologies. I am most impressed with the organization of this volume, and the extensive list of chapters contributed by expert authors from around the world who are leading the emergence of this interdisciplinary enterprise. The editors are to be commended for their skilful crafting of this important volume. The first two parts provide the basic information that is relevant to the field as a whole, the following two parts elaborate on these principles, and the last part elaborates on specific technologies or applications.
Discusses many aspects of bioreactor use and design in biotechnology. There is coverage of conventional and airlift bioreactor design, instrumentation, control and simulation of bioreactor runs, bioreactors for plant and animal culture and a descriptions of experiments.
Bioreaction engineering is fundamental to the optimization of biotechnological processes and the production of biochemicals by enzymes, microbial, plant and animal cells and higher organisms. A reference text for postgraduate students and researchers in biochemical engineering and bioreactor design, Multiphase Bioreactor Design describes the
The Textbook On Pharmaceutical Biotechnology Provides Comprehensively The Fundamental Concepts And Principles In Biotechnology To Expatiate And Substantiate Its Numerous Modern Applications With Regard To The Spectacular Development In The Pharmaceutical Industry. In A Broader Perspective, The Students Studying Biotechnology At Undergraduate And Postgraduate Levels Shall Be Grossly Benefited By Its Well-Planned Systematically Developed, Structured, Illustrated, Expanded, Elaborated, And Profusely Exemplified Subject Matter.It Essentially Comprise Five Major Chapters, Namely: Immunology And Immunological Preparations; Genetic Recombination; Antibiotics; Microbial Transformations; And Enzyme Immobilization. Besides, There Are Five Auxiliary Chapters, Namely, Advent Of Biotechnology; Biosensor Technology; Bioinformatics And Data Mining; Regulatory Issues In Biotechnology; And Safety In Biotechnology, Which Have Been Specifically Included So As To Stimulate The Students, Interest And Broaden Their Horizon Of Knowledge And Wisdom.The Authors Earnestly Believe That The Wide Coverage Of Various Topics Mentioned Above Would Certainly Render Pharmaceutical Biotechnology To Serve As An Exclusive Source Of Information S, Ideas, Inspirations Towards Research, And Finding Newer Possible Practical Solutions To Problems Encountered In The Ever Green Pasture Using Knowledge Of Biotechnology In The Pharmaceutical Industry.
The completion of the Human Genome Project and the rapid progress in cell bi- ogy and biochemical engineering, are major forces driving the steady increase of approved biotech products, especially biopharmaceuticals, in the market. Today mammalian cell products (“products from cells”), primarily monoclonals, cytokines, recombinant glycoproteins, and, increasingly, vaccines, dominate the biopharmaceutical industry. Moreover, a small number of products consisting of in vitro cultivated cells (“cells as product”) for regenerative medicine have also been introduced in the market. Their efficient production requires comprehensive knowledge of biological as well as biochemical mammalian cell culture fundamentals (e.g., cell characteristics and metabolism, cell line establishment, culture medium optimization) and related engineering principles (e.g., bioreactor design, process scale-up and optimization). In addition, new developments focusing on cell line development, animal-free c- ture media, disposables and the implications of changing processes (multi-purpo- facilities) have to be taken into account. While a number of excellent books treating the basic methods and applications of mammalian cell culture technology have been published, only little attention has been afforded to their engineering aspects. The aim of this book is to make a contribution to closing this gap; it particularly focuses on the interactions between biological and biochemical and engineering principles in processes derived from cell cultures. It is not intended to give a c- prehensive overview of the literature. This has been done extensively elsewhere.
|Author||: G. Bastin|
|Release Date||: 2013-10-22|
|ISBN 10||: 1483290980|
|Pages||: 394 pages|
This book deals with monitoring and control of biotechnological processes. Different methods are proposed which are based on the nonlinear structure of the process and do not require any a priori knowledge of the fermentation parameters. The theoretical stability and convergence properties of the proposed algorithms are analysed and their performances are illustrated by simulation results and, in many instances, by real life experiments. The concept of software sensors is introduced; these are algorithms based on the nonlinear model of the process and designed for on-line estimation of the biological variables and/or the fermentation parameters. In order to deal with process nonstationarities and parameter uncertainties, reference is made to adaptive estimation and control techniques. The book is the result of an intensive joint research effort by the authors during the last decade. It is intended as a graduate level text for students of bioengineering as well as a reference text for scientists and engineers involved in the design and optimization of bioprocesses.
Describes the state-of-the-art techniques and methods involved in the design, operation, preparation and containment of bioreactor systems, taking into account the interrelated effects of variables associated with both upstream and downstream stages of the design process. The importance of the initial steps in the development of a bioprocess, such as strain and media selection, that have an overwhelming influence on all further operations, is emphasized.;This work is intended for biochemical, chemical and bioprocess engineers; biotechnologists; industrial biochemists; micro- and molecular biologists; food scientists; and upper-level undergraduate and graduate students in these disciplines.
|Author||: Paula Rúbia Ferreira|
|Release Date||: 2017|
|Pages||: 329 pages|
In recent years, the production of hydrogen through dark fermentation has become increasingly popular because it is a sustainable approach to produce clean energy. Thus, an evaluation of studies reported on hydrogen production from different complex wastewaters will be of immense importance in economizing production technologies. This work presents a review of the advances in the bioreactor and bioprocess design for biohydrogen production from different complex wastewaters. The biohydrogen production is discussed emphasizing the production metabolic pathways, bioreactor configuration and operation, organic loading rate (OLR), pretreatment of wastewater, as well as microbial diversity. Also, in this review, various bioreactor configurations and performance parameters including H2 yield (HY) and hydrogen production rate (HPR) are evaluated and presented. The work concludes with challenges and prospects of biohydrogen production and claims for more systematic and comprehensive studies on the subject.
The knee meniscus was once thought to be a vestigial tissue, but is now known to be instrumental in imparting stability, shock absorption, load transmission, and stress distribution within the knee joint. Unfortunately, most damage to the meniscus cannot be effectively healed by the body. Meniscus tissue engineering offers a possible solution to this problem by striving to create replacement tissue that may be implanted into a defect site. With a strong focus on structure-function relationships, this book details the essential anatomical, biochemical, and mechanical aspects of this versatile tissue and reviews current meniscus tissue engineering strategies and repair techniques. We have written this text such that undergraduate students, graduate students, and researchers will find it useful as a first foray into tissue engineering, a cohesive study of the meniscus, or a reference for meniscus engineering specifications. Table of Contents: Structure-Function Relationships of the Knee Meniscus / Pathophysiology and the Need for Tissue Engineering / Tissue Engineering of the Knee Meniscus / Current Therapies and Future Directions
Bioreactor Design Fundamentals presents the development in the bioreactor field. This book discusses the applications of biological kinetics and thermodynamics. Organized into seven chapters, this book begins with an overview of the design of biological reactors that involves determining operating conditions, sizing the reactor, controlling temperature and sterility, and controlling operating variables. This text then examines the significance of pH considerations in biological reactor and process design. Other chapters consider enzyme kinetics and the equations most commonly used as models for overall enzyme kinetics. This book discusses as well the mass transfer rates in bioreactors, which are significant because of their effects on some chemical reaction rates in transformations by enzymes or by living cells. The final chapter deals with the ideal state of the continuous stirred-tank reactors (CSTRs). This book is a valuable resource for biochemical engineers and industrial microbiologists.