analytical techniques in biosciences

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Analytical Techniques in Biosciences
Author : Stanley C. Udedi,Jonathan C. Ifemeje,Chukwuebuka Egbuna,Muhammad Ajmal Shah,Kingsley C. Patrick-Iwuanyanwu,Azhar Rasul
Publisher : Academic Press
Release Date : 2021-07-15
ISBN 10 : 9780128226544
Pages : 500 pages
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Analytical Techniques in Biosciences: From Basics to Applications presents comprehensive and up-to-date information on the various analytical techniques obtainable in research laboratories across the biosciences. The chapters are grouped into two parts. Part I presents chapters that deals with the basic introductory protocols and sample preparatory guidelines for an error free analysis. This section also contains chapters that detail the various analytical techniques such as spectroscopy and mass spectrometry techniques, chromatographic techniques, electrophoretic techniques and molecular weight determination techniques. Part two presents chapters that capture various applied techniques in molecular biology. Techniques for nucleic acid assessments, polymerase chain reaction, DNA sequencing, enzyme application in genetic manipulation, and those used in immunology and Immunochemistry are presented as well. This book draws from experts from key institutions around the globe, who have simplified the chapters in a way that it will be useful to early-stage researchers as well as advanced scientists. The book is also carefully structured and integrated sequentially to aid flow, consistency and continuity. This is a must-have reference for graduate students and researchers in the biosciences. Presents basic analytical protocols, sample preparatory guidelines and conventional techniques in biosciences Details the various biochemical and molecular biology techniques including spectroscopic techniques, chromatographical techniques, centrifugation techniques and titrimetric techniques Discuses recent advances on novel techniques for screening, identification and characterization of novel molecules Describes advanced techniques such as hyphenated techniques, electroanalytical techniques, application of biosensors in biomedical research and automated methods of analysis and robotics

Bioanalytics
Author : Friedrich Lottspeich,Joachim Engels
Publisher : John Wiley & Sons
Release Date : 2018-03-26
ISBN 10 : 3527339191
Pages : 1134 pages
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Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a birdï¿1⁄2s eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology

Analytical Techniques in Biochemistry and Molecular Biology
Author : Rajan Katoch
Publisher : Springer Science & Business Media
Release Date : 2011-07-19
ISBN 10 : 9781441997852
Pages : 441 pages
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Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.

Analytical Biotechnology
Author : Thomas G.M. Schalkhammer
Publisher : Springer Science & Business Media
Release Date : 2002-08-01
ISBN 10 : 9783764365899
Pages : 331 pages
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Modern analytical biotechnology is focused on the use of a set of enabling platform technologies that provide contemporary, state-of-the-art tools for genomics, proteomics, metabolomics, drug discovery, screening, and analysis of natural product molecules. Thus, analytical biotechnology covers all areas of bioanalysis from biochips and nano-chemistry to biology and high throughput screening. Moreover, it aims to apply advanced automation and micro fabrica tion technology to the development of robotic and fluidic devices as well as integrated systems. This book focuses on enhancement technology development by promoting cross-disciplinary approaches directed toward solving key problems in biology and medicine. The scope thus brings under one umbrella many different techniques in allied areas. The purpose is to support and teach the fundamental principles and practical uses of major instrumental techniques. Major platforms are the use of immobilized molecules in biotechnology and bioanalysis, im munological techniques, immunological strip tests, fluorescence detection and confocal techniques, optical and electrochemical biosensors, biochips, micro dotting, novel transducers such as nano clusters, atomic force microscopy based techniques and analysis in complex media such as fermentation broth, plasma and serum. Techniques related to HPLC, capillary electrophoresis, gel electrophoresis, and mass spectrometry have not been included in this book but will be covered by further publications. Fundamentals in analytical biotechnology include basic and practical aspects of characterizing and analyzing DNA, proteins, and small metabolites.

Ana Techniqs in Biotechnology
Author : Goutam Bhowmik
Publisher : Tata McGraw-Hill Education
Release Date : 2011
ISBN 10 : 9780070700130
Pages : 244 pages
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'Analytical Techniques in Biotechnology' by Bhowmik and Bose offers a unique compendium of fundamental experiments, which forms the crucial foundation to understand this contemporary subject that has enormous impact on many other branches of life sciences. In addition to its simple and lucid language, the main focus of the book is to equip a beginner with the skill and ability required to conduct independent experimentation and research in laboratories. Carefully structured to cover the complete spectra of subjects under the umbrella of 'Biotechnology', this book is sure to prove a ready reference for students, faculties and researchers, all alike!

Statistical Methods in Biology
Author : S.J. Welham,S.A. Gezan,S.J. Clark,A. Mead
Publisher : CRC Press
Release Date : 2014-08-22
ISBN 10 : 1439808783
Pages : 608 pages
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Written in simple language with relevant examples, Statistical Methods in Biology: Design and Analysis of Experiments and Regression is a practical and illustrative guide to the design of experiments and data analysis in the biological and agricultural sciences. The book presents statistical ideas in the context of biological and agricultural sciences to which they are being applied, drawing on relevant examples from the authors’ experience. Taking a practical and intuitive approach, the book only uses mathematical formulae to formalize the methods where necessary and appropriate. The text features extended discussions of examples that include real data sets arising from research. The authors analyze data in detail to illustrate the use of basic formulae for simple examples while using the GenStat® statistical package for more complex examples. Each chapter offers instructions on how to obtain the example analyses in GenStat and R. By the time you reach the end of the book (and online material) you will have gained: A clear appreciation of the importance of a statistical approach to the design of your experiments, A sound understanding of the statistical methods used to analyse data obtained from designed experiments and of the regression approaches used to construct simple models to describe the observed response as a function of explanatory variables, Sufficient knowledge of how to use one or more statistical packages to analyse data using the approaches described, and most importantly, An appreciation of how to interpret the results of these statistical analyses in the context of the biological or agricultural science within which you are working. The book concludes with a guide to practical design and data analysis. It gives you the understanding to better interact with consultant statisticians and to identify statistical approaches to add value to your scientific research.

Analytical Techniques in Biotechnology
Author : Suzy Hill
Publisher : N.A
Release Date : 2016-05-26
ISBN 10 : 9781682862384
Pages : 264 pages
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Biotechnology is a rapidly progressing field of science and technology. It integrates principles and applications from diverse fields such as genomics, immunology, and many more. This book strives to equip the reader with thorough knowledge about various techniques of applied biotechnology, its applications in the areas of medicine, agriculture, environment, etc., along with details about the latest equipment and analytical tools which would help the reader gain an in-depth perspective about the subject. This book elucidates new techniques and their applications in a multidisciplinary approach and is apt for students of biotechnology and associated disciplines of study.

Cutting Edge Techniques in Biophysics, Biochemistry and Cell Biology: From Principle to Applications
Author : Neetu Mishra,Anupam Jyoti
Publisher : Bentham Science Publishers
Release Date : 2019-10-30
ISBN 10 : 9811422850
Pages : 153 pages
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Advances in biomedical research have had a profound effect on human health outcomes over the last century. Biophysical, biochemical and cellular techniques are now the backbone of modern biomedical research. Understanding these laboratory techniques is a prerequisite for investigating the processes responsible for human diseases and discovering new treatment methods. Cutting Edge Techniques in Biophysics, Biochemistry and Cell Biology: From Principle to Applications Provides information about basic and advanced analytical techniques applied in specific areas of life science and biomedical Key Features: - Book chapters present a broad overview of sophisticated analytical techniques used in biophysics, biochemistry and cell biology. - Techniques covered include in vitro cell culture techniques, flow cytometry, real time PCR, X-ray crystallography, RNA sequencing - Information about industrial and biomedical applications of techniques, (drug screening, disease models, functional assays, disease diagnosis, gene expression analysis and protein structure determination) is included. The book is an excellent introduction for students (as a textbook) and researchers (as a reference work). The information it presents will prepare readers to understand and develop research methods in life science laboratories for different projects and activities.

Principles and Techniques of Biochemistry and Molecular Biology
Author : Keith Wilson,John Walker
Publisher : Cambridge University Press
Release Date : 2010-03-04
ISBN 10 : 0521516358
Pages : 744 pages
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Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.

Surface Analysis and Techniques in Biology
Author : Vincent S. Smentkowski
Publisher : Springer Science & Business Media
Release Date : 2014-03-18
ISBN 10 : 3319013602
Pages : 326 pages
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This book summarizes the main surface analysis techniques that are being used to study biological specimens/systems. The compilation of chapters in this book highlight the benefits that surface analysis provides. The outer layer of bulk solid or liquid samples is referred to as the surface of the sample/material. At the surface, the composition, microstructure, phase, chemical bonding, electronic states, and/or texture is often different than that of the bulk material. The outer surface is where many material interactions/reactions take place. This is especially true biomaterials which may be fabricated into bio-devices and in turn implanted into tissues and organs. Surfaces of biomaterials (synthetic or modified natural materials) are of critical importance since the surface is typically the only part of the biomaterial/bio-device that comes in contact with the biological system. Analytical techniques are required to characterize the surface of biomaterials and quantify their impact in real-world biological systems. Surface analysis of biological materials started in the 1960’s and the number of researchers working in this area have increased very rapidly since then, a number of advances have been made to standard surface analytical instrumentation, and a number of new instruments have been introduced.

Instrumental Analysis in the Biological Sciences
Author : M. H. Gordon
Publisher : Springer Science & Business Media
Release Date : 2012-12-06
ISBN 10 : 1468415212
Pages : 244 pages
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Instrumental techniques of analysis have now moved from the confines of the chemistry laboratory to form an indispensable part of the analytical armoury of many workers involved in the biological sciences. It is now quite out of the question to considcr a laboratory dealing with the analysis of biological materials that is not equipped with an extensive range of instrumentation. Recent years have also seen a dramatic improvement in the ease with which such instruments can be used, and the quality and quantity of the analytical data that they can produce. This is due in no sm all part to the ubiquitous use of microprocessors and computers for instrumental control. However, under these circumstances there is areal danger of the analyst adopting a 'black box' mentality and not treating the analytical data produced in accordance with the limitations that may be inherent in the method used. Such a problem can only be overcome if the operator is fully aware of both the theoretical and instrumental constraints relevant to the technique in question. As the complexity and sheer volume of material in undergraduate courses increases, there is a tendency to reduce the amount of fundamental material that is taught prior to embarking on the more applied aspects. This is nowhere more apparent than in the teaching of instrumental techniques of analysis.

Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology
Author : Andreas Hofmann,Samuel Clokie
Publisher : Cambridge University Press
Release Date : 2018-03-31
ISBN 10 : 1107162270
Pages : 962 pages
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A major update of a best-selling textbook that introduces students to the key experimental and analytical techniques underpinning life science research.

Instrumental Analysis in the Biological Sciences
Author : M. H. Gordon
Publisher : Springer Science & Business Media
Release Date : 2012-12-06
ISBN 10 : 1468415212
Pages : 244 pages
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Instrumental techniques of analysis have now moved from the confines of the chemistry laboratory to form an indispensable part of the analytical armoury of many workers involved in the biological sciences. It is now quite out of the question to considcr a laboratory dealing with the analysis of biological materials that is not equipped with an extensive range of instrumentation. Recent years have also seen a dramatic improvement in the ease with which such instruments can be used, and the quality and quantity of the analytical data that they can produce. This is due in no sm all part to the ubiquitous use of microprocessors and computers for instrumental control. However, under these circumstances there is areal danger of the analyst adopting a 'black box' mentality and not treating the analytical data produced in accordance with the limitations that may be inherent in the method used. Such a problem can only be overcome if the operator is fully aware of both the theoretical and instrumental constraints relevant to the technique in question. As the complexity and sheer volume of material in undergraduate courses increases, there is a tendency to reduce the amount of fundamental material that is taught prior to embarking on the more applied aspects. This is nowhere more apparent than in the teaching of instrumental techniques of analysis.

Advanced Chromatographic and Electromigration Methods in BioSciences
Author : I. Mikšík,F. Tagliaro,E. Tesarová,Z. Deyl
Publisher : Elsevier
Release Date : 1998-09-28
ISBN 10 : 9780080858685
Pages : 1125 pages
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This book deals with chromatographic and electrophoretic methods applied for the separation (quantitation and identification) of biologically relevant compounds. It is assumed that the potential reader is familiar with the basics of chromatographic and electromigration methods. Individual separation modes are dealt with to an extent which follows their applicability for biomedical purposes: liquid chromatography and electromigration methods are therefore highlighted. Each chapter is completed with a list of recent literature covering the 1987-1997 period, which can be used for further guidance of the reader in his/her own field. The chapters have been written by specialists in a particular area and with an emphasis on applications to the biomedical field. This implies that theoretical and instrumental aspects are kept to a minimum which allows the reader to understand the text. Considerable attention is paid to method selection, detection and derivatization procedures and troubleshooting. The majority of examples given represent the analyses of typical naturally-occurring mixtures. Adequate attention is paid to the role of the biological matrix and sample pretreatment, and special attention is given to forensic, toxicological and clinical applications. The book is completed with an extensive Index of Compounds Separated.

Data Analysis for the Life Sciences with R
Author : Rafael A. Irizarry,Michael I. Love
Publisher : CRC Press
Release Date : 2016-10-04
ISBN 10 : 1498775861
Pages : 376 pages
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This book covers several of the statistical concepts and data analytic skills needed to succeed in data-driven life science research. The authors proceed from relatively basic concepts related to computed p-values to advanced topics related to analyzing highthroughput data. They include the R code that performs this analysis and connect the lines of code to the statistical and mathematical concepts explained.

Nuclear Analytical Methods in the Life Sciences 1994
Author : Jan Kucera,Ivan Obrusník,Enrico Sabbioni
Publisher : Springer Science & Business Media
Release Date : 2013-06-29
ISBN 10 : 1475760256
Pages : 754 pages
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Nuclear Analytical Methods in the Life Sciences •1994 is a forefront survey of key presentations from the 1993 International Conference on Nuclear Analytical Methods in the Life Sciences. Sponsored by the International Atomic Energy Agency (IAEA), this useful volume covers the spectrum of multidisciplinary research on both the methodological aspects and the development of nuclear analytical methods and their applications in the life sciences. The book is divided into six sections covering related material. These sections are: Methodology of Nuclear Analytical Methods; Environmental Applications; Biomedical Applications; Analysis of Biological Samples; Quality Assurance and Comparison with Other Methods; and a section dealing with miscellaneous issues, such as programs offered by the IAEA.

An Introduction to Statistical Analysis in Research
Author : Kathleen F. Weaver,Vanessa C. Morales,Sarah L. Dunn,Kanya Godde,Pablo F. Weaver
Publisher : John Wiley & Sons
Release Date : 2017-08-04
ISBN 10 : 1119299691
Pages : 616 pages
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Provides well-organized coverage of statistical analysis and applications in biology, kinesiology, and physical anthropology with comprehensive insights into the techniques and interpretations of R, SPSS®, Excel®, and Numbers® output An Introduction to Statistical Analysis in Research: With Applications in the Biological and Life Sciences develops a conceptual foundation in statistical analysis while providing readers with opportunities to practice these skills via research-based data sets in biology, kinesiology, and physical anthropology. Readers are provided with a detailed introduction and orientation to statistical analysis as well as practical examples to ensure a thorough understanding of the concepts and methodology. In addition, the book addresses not just the statistical concepts researchers should be familiar with, but also demonstrates their relevance to real-world research questions and how to perform them using easily available software packages including R, SPSS®, Excel®, and Numbers®. Specific emphasis is on the practical application of statistics in the biological and life sciences, while enhancing reader skills in identifying the research questions and testable hypotheses, determining the appropriate experimental methodology and statistical analyses, processing data, and reporting the research outcomes. In addition, this book: • Aims to develop readers’ skills including how to report research outcomes, determine the appropriate experimental methodology and statistical analysis, and identify the needed research questions and testable hypotheses • Includes pedagogical elements throughout that enhance the overall learning experience including case studies and tutorials, all in an effort to gain full comprehension of designing an experiment, considering biases and uncontrolled variables, analyzing data, and applying the appropriate statistical application with valid justification • Fills the gap between theoretically driven, mathematically heavy texts and introductory, step-by-step type books while preparing readers with the programming skills needed to carry out basic statistical tests, build support figures, and interpret the results • Provides a companion website that features related R, SPSS, Excel, and Numbers data sets, sample PowerPoint® lecture slides, end of the chapter review questions, software video tutorials that highlight basic statistical concepts, and a student workbook and instructor manual An Introduction to Statistical Analysis in Research: With Applications in the Biological and Life Sciences is an ideal textbook for upper-undergraduate and graduate-level courses in research methods, biostatistics, statistics, biology, kinesiology, sports science and medicine, health and physical education, medicine, and nutrition. The book is also appropriate as a reference for researchers and professionals in the fields of anthropology, sports research, sports science, and physical education. KATHLEEN F. WEAVER, PhD, is Associate Dean of Learning, Innovation, and Teaching and Professor in the Department of Biology at the University of La Verne. The author of numerous journal articles, she received her PhD in Ecology and Evolutionary Biology from the University of Colorado. VANESSA C. MORALES, BS, is Assistant Director of the Academic Success Center at the University of La Verne. SARAH L. DUNN, PhD, is Associate Professor in the Department of Kinesiology at the University of La Verne and is Director of Research and Sponsored Programs. She has authored numerous journal articles and received her PhD in Health and Exercise Science from the University of New South Wales. KANYA GODDE, PhD, is Assistant Professor in the Department of Anthropology and is Director/Chair of Institutional Review Board at the University of La Verne. The author of numerous journal articles and a member of the American Statistical Association, she received her PhD in Anthropology from the University of Tennessee. PABLO F. WEAVER, PhD, is Instructor in the Department of Biology at the University of La Verne. The author of numerous journal articles, he received his PhD in Ecology and Evolutionary Biology from the University of Colorado.

Quantitative Investigations in the Biosciences using MINITAB
Author : John Eddison
Publisher : CRC Press
Release Date : 1999-12-20
ISBN 10 : 9781584880332
Pages : 472 pages
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Until recently, acquiring a background in the basic methodological principles that apply to most types of investigations meant struggling to obtain results through laborious calculations. The advent of statistical software packages has removed much of the tedium and many of the errors of manual calculations and allowed a marked increase in the depth and sophistication of analyses. Although most statistics classes now incorporate some instruction in using a statistics package, most introductory texts do not. Quantitative Investigations in the Biosciences using MINITAB fills this void by providing an introduction to investigative methods that, in addition to outlining statistical principles and describing methods of calculations, also presents essential commands and interprets output from the statistics package MINITAB. The author introduces the three basic elements of investigations-design, analysis, and reporting-using an extremely accessible approach that keeps mathematical detail to a minimum. He groups statistical tests according to the type of problem they are used to examine, such as comparisons, sequential relationships, and associations. Quantitative Investigations in the Biosciences using MINITAB draws techniques and examples from a variety of subjects, ranging from physiology and biochemistry through to ecology, behavioral sciences, medicine, agriculture and horticulture, and complements the mathematical results with formal conclusions for all of the worked examples. It thus provides an ideal handbook for anyone in virtually any field who wants to apply statistical techniques to their investigations.

Biomolecular and Bioanalytical Techniques
Author : Vasudevan Ramesh
Publisher : Wiley
Release Date : 2019-05-13
ISBN 10 : 1119483964
Pages : 576 pages
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An essential guide to biomolecular and bioanalytical techniques and their applications Biomolecular and Bioanalytical Techniques offers an introduction to, and a basic understanding of, a wide range of biophysical techniques. The text takes an interdisciplinary approach with contributions from a panel of distinguished experts. With a focus on research, the text comprehensively covers a broad selection of topics drawn from contemporary research in the fields of chemistry and biology. Each of the internationally reputed authors has contributed a single chapter on a specific technique. The chapters cover the specific technique’s background, theory, principles, technique, methodology, protocol and applications. The text explores the use of a variety of analytical tools to characterise biological samples. The contributors explain how to identify and quantify biochemically important molecules, including small molecules as well as biological macromolecules such as enzymes, antibodies, proteins, peptides and nucleic acids. This book is filled with essential knowledge and explores the skills needed to carry out the research and development roles in academic and industrial laboratories. A technique-focused book that bridges the gap between an introductory text and a book on advanced research methods Provides the necessary background and skills needed to advance the research methods Features a structured approach within each chapter Demonstrates an interdisciplinary approach that serves to develop independent thinking Written for students in chemistry, biological, medical, pharmaceutical, forensic and biophysical sciences, Biomolecular and Bioanalytical Techniques is an in-depth review of the most current biomolecular and bioanalytical techniques in the field.

Advances in Biological Science Research
Author : Surya Nandan Meena,Milind Naik
Publisher : Academic Press
Release Date : 2019-05-17
ISBN 10 : 0128174986
Pages : 580 pages
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Advances in Biological Science Research: A Practical Approach provides discussions on diverse research topics and methods in the biological sciences in a single platform. This book provides the latest technologies, advanced methods, and untapped research areas involved in diverse fields of biological science research such as bioinformatics, proteomics, microbiology, medicinal chemistry, and marine science. Each chapter is written by renowned researchers in their respective fields of biosciences and includes future advancements in life science research. Discusses various research topics and methods in the biological sciences in a single platform Comprises the latest updates in advanced research techniques, protocols, and methods in biological sciences Incorporates the fundamentals, advanced instruments, and applications of life science experiments Offers troubleshooting for many common problems faced while performing research experiments