a laboratory guide to the tight junction

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A Laboratory Guide to the Tight Junction
Author : Jianghui Hou
Publisher : Academic Press
Release Date : 2020-04-24
ISBN 10 : 0128226439
Pages : 420 pages
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A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models. Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches for tissue fixation and preparation. Chapter 5 discusses Light microscopy, while chapter 6 presents Electron microscopic approaches. Chapter 7 covers Transgenic manipulation in cell cultures, including DNA and siRNA, Mutagenesis, and viral infection. Chapter 8 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and overexpression. The final chapter discusses the future developments of new approaches for tight junction research. Researchers and advanced students in bioscience working on topics of cell junction, ion channel and membrane protein will benefit from the described methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical characterization of tight junctions in organ systems, and their connection to human diseases. Provides consistent and detailed research methods Covers various cell, tissue and animal models Includes step-by-step guidance from beginner to sophisticated levels

A Laboratory Guide to Human Physiology
Author : Stuart Ira Fox
Publisher : N.A
Release Date : 1984
ISBN 10 : 9780697047243
Pages : 323 pages
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Oral Histology Laboratory Guide
Author : N.A
Publisher : N.A
Release Date : 1978
ISBN 10 :
Pages : 73 pages
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A Laboratory Guide to the Mammalian Embryo
Author : David K. Gardner,Michelle Lane,Andrew J. Watson
Publisher : Oxford University Press
Release Date : 2004-01-08
ISBN 10 : 9780198032519
Pages : 432 pages
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This book pulls together the full range of cell culture, biochemical, microscopic, and genetic techniques to study the early mammalian embryo. Until now, there has never been such a comprehensive compendium, though there have been more focused books of protocol, such as Manipulating the Mouse Embryo, from Cold Spring Harbor. This book is intended to appeal to all constituencies, from basic experimental science to clinical and animal science applications.

American Journal of Physiology
Author : N.A
Publisher : N.A
Release Date : 1992
ISBN 10 :
Pages : 329 pages
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"The William Townsend Porter memorial volume": v. 158.

The Paracellular Channel
Author : Jianghui Hou
Publisher : Academic Press
Release Date : 2018-06-08
ISBN 10 : 0128146362
Pages : 248 pages
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The Paracellular Channel: Biology, Physiology and Disease serves as the first volume to offer a cohesive and unifying picture of the critical functions of paracellular channels (tight junctions) in different tissues. This new class of ion channel utilizes a completely different mechanism to create ion passage pathways across the cell junction. This volume outlines common principles that govern the organization and regulation of these diverse cellular structures, describes the methodology of study, and highlights the pathophysiologic consequence of abnormal structure and functions of the paracellular channels in human diseases. Coverage includes biochemical, biophysical, structural, physiologic analyses of the paracellular channel, and new technologies for recording and characterization. Offers integrated coverage of all key aspects of the paracellular channel, an understudied field that may hold key insights into some of the most mysterious aspects of physiology Targets different levels of expertise, spanning from graduate students, interns and clinical fellows, to seasoned researchers that study functions, regulation and dysfunctions of different tissue barriers Provides a cohesive and unifying picture that describes the critical functions of paracellular channels (tight junctions) in different tissues

Skin Barrier Function
Author : T. Agner
Publisher : Karger Medical and Scientific Publishers
Release Date : 2016-02-04
ISBN 10 : 3318055867
Pages : 164 pages
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Although a very fragile structure, the skin barrier is probably one of the most important organs of the body. Inward/out it is responsible for body integrity and outward/in for keeping microbes, chemicals, and allergens from penetrating the skin. Since the role of barrier integrity in atopic dermatitis and the relationship to filaggrin mutations was discovered a decade ago, research focus has been on the skin barrier, and numerous new publications have become available. This book is an interdisciplinary update offering a wide range of information on the subject. It covers new basic research on skin markers, including results on filaggrin and on methods for the assessment of the barrier function. Biological variation and aspects of skin barrier function restoration are discussed as well. Further sections are dedicated to clinical implications of skin barrier integrity, factors influencing the penetration of the skin, influence of wet work, and guidance for prevention and saving the barrier. Distinguished researchers have contributed to this book, providing a comprehensive and thorough overview of the skin barrier function. Researchers in the field, dermatologists, occupational physicians, and related industry will find this publication an essential source of information.

Alternatives to Laboratory Animals
Author : N.A
Publisher : N.A
Release Date : 1990
ISBN 10 :
Pages : 329 pages
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Laboratory Guide in Pharmacology
Author : Tom S. Miya,Harald Groth Oxholm Holck
Publisher : Burgess International Group Incorporated
Release Date : 1973
ISBN 10 :
Pages : 136 pages
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Laboratory Guide in Pharmacology
Author : Harald Groth Oxholm Holck,Tom Saburo Miya
Publisher : N.A
Release Date : 1968
ISBN 10 :
Pages : 163 pages
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A Laboratory Guide to Human Physiology
Author : Stuart Ira Fox
Publisher : N.A
Release Date : 1976
ISBN 10 : 9780697045447
Pages : 323 pages
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Instructors Guide to Text and Media
Author : William Germann,WILLIAM
Publisher : N.A
Release Date : 2001-12
ISBN 10 : 9780805356694
Pages : 329 pages
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